Recombinant Human ALOX12B protein, His & T7-tagged

• Cat.No.: ALOX12B-7852H
• Product Overview: Recombinant Human ALOX12B aa. (Leu479~Ile701 (Accession # O75342)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene are associated with nonbullous congenital ichthyosiform erythroderma.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.6kDa
• Protein length: Leu479~Ile701 (Accession # O75342)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: ALOX12B arachidonate 12-lipoxygenase, 12R type [ Homo sapiens (human) ]
• Official Symbol: ALOX12B
• Synonyms: ALOX12B; arachidonate 12-lipoxygenase, 12R type; ARCI2; 12R-LOX; arachidonate 12-lipoxygenase, 12R-type; 12R-lipoxygenase; epidermis-type lipoxygenase 12
• Gene ID: 242
• mRNA Refseq: NM_001139.2
• Protein Refseq: NP_001130.1
• UniProt ID: O75342

Reviews

06/22/2022

ALOX12B has good absorbability and high bioavailability.

01/29/2021

Highly effective in signal transduction pathways.

08/20/2020

Under different environmental conditions,ALOX12B showed good stability.

Q&As

How does ALOX12B gene mutation affect cardiovascular health?

Mutations in the ALOX12B gene may lead to the development of cardiovascular disease, which can affect cardiovascular health.

Are ALOX12B gene variants associated with tumors?

The ALOX12B gene variant has not been shown to be associated with tumors.

Based on the mutation of ALOX12B gene, is there an effective treatment at present?

There is no specific treatment for the ALOX12B gene mutation. Treatment is mostly based on symptom relief and reduction of disease progression.

How is the ALOX12B gene mutant diagnosed?

A mutation of the ALOX12B gene can be diagnosed with a genetic test or blood test.

What is the regulatory mechanism of ALOX12B gene?

The regulatory mechanisms of ALOX12B gene include transcription factors, miRNA and other regulatory mechanisms.

Does ALOX12B gene mutation have an impact on skin health?

Mutations in the ALOX12B gene may lead to the development of skin diseases that affect skin health.

Does ALOX12B gene mutation cause liver disease?

ALOX12B gene mutation and liver disease has not been proven to have a clear link.

In which populations is the ALOX12B gene most common?

Current studies have shown that the ALOX12B variant is more common in Asian populations.

What is the function of the ALOX12B gene?

ALOX12B encodes the 12R-LOX enzyme, which is involved in biological processes such as lipid metabolism and inflammatory responses.

How do polymorphisms in the ALOX12B gene affect its function?

The polymorphism of ALOX12B gene can affect the activity of the enzyme it encodes, and thus affect related biological processes.

Is the SNP of ALOX12B gene related to drug metabolism?

The SNP of ALOX12B gene has not been confirmed to have a clear association with drug metabolism.

Does ALOX12B affect the immune system?

The ALOX12B gene plays an important role in the body's inflammatory response and therefore may influence the function of the immune system.

Which diseases are related to the expression of ALOX12B gene?

Mutations in the ALOX12B gene have been linked to skin disorders, asthma, cardiovascular disease and more.

Does ALOX12B gene affect body metabolism?

The ALOX12B gene can affect biological processes such as fat metabolism in the body, thus affecting body metabolism.

Which diseases are the ALOX12B gene mutations clearly associated with?

A mutant version of the ALOX12B gene has been linked to a rare inherited fat metabolism disorder called Allen's syndrome.

In which organs is ALOX12B gene more expressed?

ALOX12B gene is more expressed in skin, liver, heart, kidney and other organs.