Recombinant Human ALPP protein, GST-tagged

• Cat.No.: ALPP-30162H
• Product Overview: Recombinant Human ALPP (199-535 aa) protein, fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization.
• Protein length: Ser199-Pro535
• AA Sequence: SARQEGCQDIATQLISNMDIDVILG GGRKYMFRMGTPDPEYPDDYSQGGT RLDGKNLVQEWLAKRQGARYVWNRT ELMQASLDPSVTHLMGLFEPGDMKY EIHRDSTLDPSLMEMTEAALRLLSR NPRGFFLFVEGGRIDHGHHESRAYR ALTETIMFDDAIERAGQLTSEEDTL SLVTADHSHVFSFGGYPLRGSSIFG LAPGKARDRKAYTVLLYGNGPGYVL KDGARPDVTESESGSPEYRQQSAVP LDEETHAGEDVAVFARGPQAHLVHG VQEQTFIAHVMAFAACLEPYTACDL APPAGTTDAAHPGRSVVPALLPLLA GTLLLLETATAP
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Stability: Store for up to 12 months at -20°C to -80°C as lyophilized powder.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.

Gene Information

• Gene Name: ALPP alkaline phosphatase, placental [ Homo sapiens ]
• Official Symbol: ALPP
• Synonyms: ALPP; alkaline phosphatase, placental; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; Regan isozyme; PLAP-1; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme; ALP; PALP; PLAP; FLJ61142
• Gene ID: 250
• mRNA Refseq: NM_001632
• Protein Refseq: NP_001623
• MIM: 171800
• UniProt ID: P05187

Reviews

12/01/2020

High-throughput screening compatibility.

11/05/2018

Consistent protein expression analysis.

10/25/2017

Precise protein quantification in ELISA.

Q&As

Can ALPP serve as a diagnostic biomarker or therapeutic target for specific diseases?

ALPP may serve as a diagnostic biomarker for specific diseases, allowing for disease detection and monitoring, and its targeting could hold therapeutic potential in certain pathological conditions.

How does ALPP contribute to the metabolism and turnover of its substrate(s) in physiological and pathological conditions?

ALPP participates in the metabolism and turnover of its substrate(s), including the hydrolysis of phosphoester bonds and the regulation of cellular phosphate homeostasis.

What are the downstream signaling pathways and molecular interactions regulated by ALPP in cellular functions?

ALPP regulates downstream signaling pathways and molecular interactions involved in cellular processes like phosphate metabolism, intracellular signaling, and extracellular matrix remodeling.

What are the regulatory mechanisms controlling ALPP gene expression and enzyme activity?

ALPP gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in cellular homeostasis and disease processes.

What is the subcellular localization and trafficking of ALPP within cells?

ALPP is localized to specific cellular compartments, such as the plasma membrane, endoplasmic reticulum, or lysosomes, and its trafficking may be regulated by sorting signals and intracellular transport machinery.

Can ALPP activity be modulated by specific factors or inhibitors, and how does this impact cellular processes?

ALPP activity can be modulated by factors such as pH, calcium, and inhibitors, which can affect its catalytic efficiency and cellular functions.

How does ALPP deficiency or dysregulation contribute to the pathogenesis of diseases and disorders?

ALPP deficiency or dysregulation is associated with various diseases, including metabolic disorders, bone diseases, and certain cancers, highlighting its pathological implications.

How can ALPP activity and function be manipulated to modulate cellular processes and disease outcomes?

Manipulating ALPP activity and function could offer strategies to modulate cellular processes and disease outcomes, potentially leading to therapeutic interventions and improved patient outcomes.

How does ALPP expression and activity change during development, differentiation, and disease progression?

ALPP expression and activity exhibit dynamic changes during development, cellular differentiation, and disease progression, reflecting its roles in different physiological and pathological contexts.

What is the expression pattern of ALPP in different tissues and cell types?

ALPP shows a tissue-specific expression pattern, with higher levels in tissues such as liver, bone, and kidney, and lower levels in other organs.