Recombinant Human ANP, His-tagged, GST-tagged

• Cat.No.: ANP-3768H
• Product Overview: Atrial Natriuretic Peptide (ANP)

Specification

• Description: The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6.
• Source: E. Coli
• Species: Human
• Tag: His,GST
• Form: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 42.7kDa
• Protein length: 151
• Endotoxin: <1.0eu per 1ug (determined by the lal</1.0eu
• Purity: >95%
• Applications: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Notes: Small volumes of ANP recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ANP
• Official Symbol: ANP
• Synonyms: NPPA; NPPA; ANF; ANP; PND; ATFB6; CDD-AN...
• Gene ID: 4878
• mRNA Refseq: NM_006172.3
• Protein Refseq: NP_006163.1
• MIM: 108780
• UniProt ID: P01160
• Chromosome Location: Chromosome: 1; NC_000001.10 (11905766..11907840, complement). Location: 1p36.21
• Function: in cardiovascular homeostasis through regulation of natriuresis, diuresis,and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3

Reviews

06/19/2021

The ANP protein is of exceptional quality, making it an ideal choice to meet my experimental needs.

11/29/2019

The ANP protein is highly recommended for researchers conducting protein studies, especially in Western blotting (WB) experiments.

10/29/2018

This protein demonstrates great performance in WB assays, providing accurate and reliable results that are crucial for protein analysis.

04/06/2018

the ANP protein has proven to be an invaluable tool in protein electron microscopy (EM) structure analysis.

Q&As

Are there any genetic variations or mutations associated with ANP protein?

Yes, certain genetic variations in the ANP gene have been identified. These variations can affect the expression or function of ANP protein and have been implicated in cardiovascular diseases like hypertension and heart failure. However, more studies are needed to fully understand the impact of these genetic variations on ANP physiology.

How is ANP protein cleared from the body?

ANP protein is primarily cleared by the kidneys. It is filtered through the glomeruli and then partially reabsorbed in the proximal tubules. The remaining ANP is excreted in the urine.

How is ANP protein synthesized and secreted?

ANP protein is synthesized and secreted primarily by the atrial myocytes in the heart. The synthesis process involves the translation of the ANP mRNA, which is transcribed from the ANP gene. The pre-proANP molecule is then produced, which undergoes post-translational modifications, including cleavage of the signal peptide to form pro-ANP. Pro-ANP is further processed in secretory granules within the atrial myocytes, resulting in the formation of the mature ANP peptide.

How is ANP protein released?

ANP protein is released in response to increased pressure and stretching of the heart's atria, usually caused by excess blood volume. When the heart senses this stretching, it secretes ANP into the bloodstream. ANP production is regulated by various factors like atrial filling, sympathetic nervous system activity, and hormonal influences.

Can ANP levels be used as a diagnostic marker?

Yes, ANP levels can be used as a diagnostic marker in certain clinical situations. Elevated levels of ANP may indicate conditions such as heart failure, atrial fibrillation, atrial enlargement, or renal dysfunction. Conversely, low levels of ANP can be seen in conditions associated with low blood volume, such as dehydration or hypovolemia.

Are there any clinical implications of ANP protein?

Yes, ANP protein is clinically relevant. It is often measured as a biomarker in conditions like heart failure, where ANP levels are elevated due to increased cardiac strain. ANP tests can assist in diagnosing and monitoring heart-related disorders. Additionally, ANP-based medications (such as Nesiritide) have been used in managing acute decompensated heart failure.

Is ANP protein involved in blood pressure regulation?

Yes, ANP protein plays a crucial role in blood pressure regulation. By promoting sodium excretion, ANP reduces blood volume, which in turn decreases blood pressure. ANP also acts as a vasodilator, relaxing and widening blood vessels, further contributing to the lowering of blood pressure.

What factors influence ANP protein secretion?

ANP protein secretion is primarily influenced by the stretching of the atria. Increased atrial pressure, caused by factors like volume overload or hypertension, stimulates the release of ANP. Other factors that can affect ANP secretion include sympathetic nervous system activity, atrial distention, atrial stretch receptors, and hormonal influences.