Recombinant Rabbit ANP protein, His-tagged

• Cat.No.: ANP-3344R
• Product Overview: Recombinant Rabbit ANP protein(Ser124~Tyr151), fused with N-terminal His Tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Rabbit
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 33kDa
• Protein Length: Ser124~Tyr151
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Reviews

06/19/2021

The ANP protein is of exceptional quality, making it an ideal choice to meet my experimental needs.

11/29/2019

The ANP protein is highly recommended for researchers conducting protein studies, especially in Western blotting (WB) experiments.

10/29/2018

This protein demonstrates great performance in WB assays, providing accurate and reliable results that are crucial for protein analysis.

04/06/2018

the ANP protein has proven to be an invaluable tool in protein electron microscopy (EM) structure analysis.

Q&As

Are there any genetic variations or mutations associated with ANP protein?

Yes, certain genetic variations in the ANP gene have been identified. These variations can affect the expression or function of ANP protein and have been implicated in cardiovascular diseases like hypertension and heart failure. However, more studies are needed to fully understand the impact of these genetic variations on ANP physiology.

How is ANP protein cleared from the body?

ANP protein is primarily cleared by the kidneys. It is filtered through the glomeruli and then partially reabsorbed in the proximal tubules. The remaining ANP is excreted in the urine.

How is ANP protein synthesized and secreted?

ANP protein is synthesized and secreted primarily by the atrial myocytes in the heart. The synthesis process involves the translation of the ANP mRNA, which is transcribed from the ANP gene. The pre-proANP molecule is then produced, which undergoes post-translational modifications, including cleavage of the signal peptide to form pro-ANP. Pro-ANP is further processed in secretory granules within the atrial myocytes, resulting in the formation of the mature ANP peptide.

How is ANP protein released?

ANP protein is released in response to increased pressure and stretching of the heart's atria, usually caused by excess blood volume. When the heart senses this stretching, it secretes ANP into the bloodstream. ANP production is regulated by various factors like atrial filling, sympathetic nervous system activity, and hormonal influences.

Can ANP levels be used as a diagnostic marker?

Yes, ANP levels can be used as a diagnostic marker in certain clinical situations. Elevated levels of ANP may indicate conditions such as heart failure, atrial fibrillation, atrial enlargement, or renal dysfunction. Conversely, low levels of ANP can be seen in conditions associated with low blood volume, such as dehydration or hypovolemia.

Are there any clinical implications of ANP protein?

Yes, ANP protein is clinically relevant. It is often measured as a biomarker in conditions like heart failure, where ANP levels are elevated due to increased cardiac strain. ANP tests can assist in diagnosing and monitoring heart-related disorders. Additionally, ANP-based medications (such as Nesiritide) have been used in managing acute decompensated heart failure.

Is ANP protein involved in blood pressure regulation?

Yes, ANP protein plays a crucial role in blood pressure regulation. By promoting sodium excretion, ANP reduces blood volume, which in turn decreases blood pressure. ANP also acts as a vasodilator, relaxing and widening blood vessels, further contributing to the lowering of blood pressure.

What factors influence ANP protein secretion?

ANP protein secretion is primarily influenced by the stretching of the atria. Increased atrial pressure, caused by factors like volume overload or hypertension, stimulates the release of ANP. Other factors that can affect ANP secretion include sympathetic nervous system activity, atrial distention, atrial stretch receptors, and hormonal influences.