Recombinant Human AOC2 293 Cell Lysate

• Cat.No.: AOC2-8823HCL

Specification

• Description: Antigen standard for amine oxidase, copper containing 2 (retina-specific) (AOC2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: AOC2 amine oxidase, copper containing 2 (retina-specific) [ Homo sapiens ]
• Official Symbol: AOC2
• Synonyms: AOC2; amine oxidase, copper containing 2 (retina-specific); retina-specific copper amine oxidase; DAO2; RAO; SSAO; semicarbazide-sensitive amine oxidase
• Gene ID: 314
• mRNA Refseq: NM_009590
• Protein Refseq: NP_033720
• MIM: 602268
• UniProt ID: O75106
• Chromosome Location: 17q21
• Pathway: Glycine, serine and threonine metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Phenylalanine metabolism, organism-specific biosystem; Phenylalanine metabolism, conserved biosystem; Tyrosine metabolism, organism-specific biosystem; Tyrosine metabolism, conserved biosystem
• Function: aliphatic-amine oxidase activity; aminoacetone:oxygen oxidoreductase(deaminating) activity; copper ion binding; electron carrier activity; metal ion binding; oxidoreductase activity; phenethylamine:oxygen oxidoreductase (deaminating) activity; primary amine oxidase activity; quinone binding; tryptamine:oxygen oxidoreductase (deaminating) activity

Reviews

10/19/2022

Immunogenicity of AOC2 is very weak, does not cause an immune response, and is very safe.

06/12/2022

The catalytic activity of AOC2 is very excellent, and it can be widely used in various chemical reactions, and has high practical value.

02/12/2021

Repeatability is very good, can reduce unnecessary repeated experiments, improve the efficiency of the experiment.

Q&As

What is the application prospect of AOC2 ?

AOC2 has important application potential in many fields such as biological science and medical field, and has a very wide application prospect.

What are the potential effects of AOC2 in clinical diseases?

AOC2 has potential therapeutic effects in the treatment of kidney disease and cancer.

How to apply AOC2 in tumor therapy?

At present, the specific application of AOC2 in tumor therapy still needs to be further studied.

Does the clinical research results of AOC2 in the treatment of tumor?

At present, the clinical study of AOC2 in the treatment of tumor has not been conclusive, and further research is needed.

How to adjust the dose of AOC2 ?

The dose of AOC2 needs to be adjusted individually according to the clinical situation. In general, use strictly according to the doctor's advice.

Is the production technology of AOC2 mature?

Yes, some mature production technologies have been developed and the corresponding processes have been applied on a large scale.

What are the properties of AOC2 ?

AOC2 is a recombinant human protein from exons, and its structure and function conform to physiological characteristics.

Which the factors affecting human health of AOC2 ?

AOC2 has been implicated in the occurrence and development of certain kidney diseases, as well as in the occurrence and development of tumors.

What are the similarities and differences between AOC2 and other biosimilar products?

AOC2 has higher biological activity and stability compared with other similar biological products.

Is there a renewable source of AOC2 ?

Yes, AOC2 can be mass-produced through cell culture, genetic engineering and other technologies.

Has AOC2 been approved by the national regulatory authority?

It is necessary to go through the relevant approval process to obtain the approval of the national regulatory authority for use.

How to solve the technical problems encountered in the production process of AOC2 ?

It is necessary to solve relevant technical problems by strengthening research and developing new process technologies.

What is the specific scope of use of AOC2 ?

AOC2 is used in many fields such as kidney disease treatment and tumor treatment.

What are the clinical research results of AOC2 in the treatment of kidney disease?

AOC2 can alleviate physiological processes such as renal fibrosis and inflammatory response, and has a potential role in the prevention and treatment of kidney diseases.

Does AOC2 have an impact on human reproductive health?

No studies have shown that AOC2 has adverse effects on human reproductive health.

What is the action principle of AOC2 in the treatment of kidney diseases and tumors?

The regulation of AOC2 on intracellular oxygen molecules is related to anti-oxidative stress, and can also affect cell cycle, proliferation and apoptosis.

Does the use of AOC2 require clinical trials?

Yes, AOC2 needs to undergo relevant clinical trials to evaluate its efficacy and safety.

Are there side effects of AOC2 ?

Recombinant AOC2 proteins may have some side effects, such as headache, nausea, pain, etc., but they are relatively rare.