Recombinant Human APEX2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: APEX2-3015H
• Product Overview: APEX2 MS Standard C13 and N15-labeled recombinant protein (NP_055296) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 57.4 kDa
• AA Sequence: MLRVVSWNINGIRRPLQGVANQEPS NCAAVAVGRILDELDADIVCLQETK VTRDALTEPLAIVEGYNSYFSFSRN RSGYSGVATFCKDNATPVAAEEGLS GLFATQNGDVGCYGNMDEFTQEELR ALDSEGRALLTQHKIRTWEGKEKTL TLINVYCPHADPGRPERLVFKMRFY RLLQIRAEALLAAGSHVIILGDLNT AHRPIDHWDAVNLECFEEDPGRKWM DSLLSNLGCQSASHVGPFIDSYRCF QPKQEGAFTCWSAVTGARHLNYGSR LDYVLGDRTLVIDTFQASFLLPEVM GSDHCPVGAVLSVSSVPAKQCPPLC TRFLPEFAGTQLKILRFLVPLEQSP VLEQSTLQHNNQTRVQTCQNKAQVR STRPQPSQVGSSRGQKNLKSYFQPS PSCPQASPDIELPSLPLMSALMTPK TPEEKAVAKVVKGQAKTSEAKDEKE LRTSFWKSVLAGPLRTPLCGGHREP CVMRTVKKPGPNLGRRFYMCARPRG PPTDPSSRCNFFLWSRPSTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: APEX2 apurinic/apyrimidinic endodeoxyribonuclease 2 [ Homo sapiens (human) ]
• Official Symbol: APEX2
• Synonyms: APEX2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; DNA-(apurinic or apyrimidinic site) lyase 2; APE2; APEXL2; XTH2; APEX nuclease 2; AP endonuclease 2; AP endonuclease XTH2; APEX nuclease-like 2; apurinic-apyrimidinic endonuclease 2; DNA-apurinic or apyrimidinic site lyase 2; apurinic/apyrimidinic endonuclease-like 2
• Gene ID: 27301
• mRNA Refseq: NM_014481
• Protein Refseq: NP_055296
• MIM: 300773
• UniProt ID: Q9UBZ4

Reviews

11/08/2021

APEX2 produced by them has high expression and easy detection.

03/29/2021

Maximizes the use of energy and resources by optimizing the production process and equipment.

05/23/2020

The results are very consistent in each experiment or application, thanks to its high stability and reproducibility, which provides great convenience for follow-up studies.

Q&As

What is the detection principle of APEX2 ?

The APEX2 enables the detection of proteins by producing oxidation products and forming a stain in a visible range.

What are the advantages of APEX2 s compared with other labeling techniques?

Recombinant APEX2 proteins are more sensitive and stable than other labeling technologies, allowing for labeling and detection in a shorter time.

What are the advantages of APEX2 ?

APEX2 has the characteristics of high sensitivity, high specificity and rapid response, which is suitable for a variety of research and therapeutic needs.

How to prepare APEX2 ?

APEX2 can be expressed and purified by genetic engineering technology.

What is the mechanism of action of APEX2 ?

The APEX2 applies detectable markers to the protein through oxidation reactions, enabling visual study of the target protein.

What is the research progress of APEX2 in cancer field?

Recombinant APEX2 protein can be used as a tumor marker to help realize early diagnosis, treatment monitoring and prognosis assessment of cancer.

How to evaluate the effect of APEX2 ?

The effect of APEX2 s can usually be evaluated by comparing markers such as labeling effect, specificity, and sensitivity.

What are the limitations of APEX2 ?

The recombinant APEX2 protein may have errors under certain conditions due to its special oxidation reaction mechanism.

In addition to the field of cancer, does APEX2 have applications in other disease research?

Yes, APEX2 also has application potential in neurodegenerative diseases, cardiovascular diseases and other fields.

What are the clinical applications of APEX2 ?

The recombinant APEX2 protein can be used clinically in the fields of labeling targeted drugs, tumor therapy and biosensors.