Recombinant Human APIP 293 Cell Lysate

• Cat.No.: APIP-8795HCL

Specification

• Description: Antigen standard for APAF1 interacting protein (APIP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: APIP APAF1 interacting protein [ Homo sapiens ]
• Official Symbol: APIP
• Synonyms: APIP; APAF1 interacting protein; probable methylthioribulose-1-phosphate dehydratase; APIP2; CGI 29; Mmrp19; MTRu-1-P dehydratase; APAF1-interacting protein; CGI29; CGI-29; MMRP19; dJ179L10.2
• Gene ID: 51074
• mRNA Refseq: NM_015957
• Protein Refseq: NP_057041
• MIM: 612491
• UniProt ID: Q96GX9
• Chromosome Location: 11p13
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Metabolism of polyamines, organism-specific biosystem; Methionine salvage pathway, organism-specific biosystem
• Function: identical protein binding; ion binding; lyase activity; metal ion binding; methylthioribulose 1-phosphate dehydratase activity; protein binding

Reviews

12/03/2022

Experimenting with this Apip allows me to focus more on experimental design and data analysis without worrying about reproducibility of experimental results.

06/19/2021

Production process of Apip is an environmental model and sets an example for the field of green production.

06/28/2020

Under the action of various chemical agents or drugs, the stability of this protein is still very good, indicating that it has excellent anti-interference ability.

04/11/2020

The lower limit of detection of Apip in Western blot is very low, and low concentration of target protein can be detected, indicating that it has the characteristics of high sensitivity and low detection limit.

05/23/2019

This short half-life and high clearance characteristics make Apip have greater potential in the treatment of tumors, infections and other diseases.

Q&As

Does Apip produce immunogenicity?

The recombinant Apip protein is low in immunogenicity and has not been found to produce any immune response to the body.

What is the application prospect of Apip in drug development?

The recombinant Apip protein has potential applications in drug development and can be used to make drugs for the treatment of platelet dysfunction.

What are the physical and chemical properties of Apip ?

The physicochemical properties of Apip are a very stable protein that can adapt to a variety of pH values and temperatures.

Which diseases can Apip be used to treat?

Apip protein can be used to treat platelet dysfunction, influenza virus and other related diseases.

How is Apip used in diagnosis?

The Apip can be used to diagnose influenza virus infection by detecting the level of antibodies to the Apip .

What is the mechanism of action of Apip in the treatment of platelet dysfunction?

Recombinant Apip protein can enhance the adhesion of platelets by activating the polymers on the surface of platelets, thus treating platelet dysfunction.

How is Apip prepared?

First, the target gene needs to be cloned into the expression vector, and then expressed in E. coli and other hosts, and the recombinant Apip protein can be obtained after purification and refolding.

Recombinant Apip protein can be used to make drugs for the treatment of platelet dysfunction. What is its effect?

The recombinant Apip protein has been clinically tested in patients as a drug for the treatment of platelet dysfunction, and the results show that it has some efficacy in the treatment of this disease.

Can Apip be degraded by digestive enzymes?

Apip can maintain relative stability under the action of digestive enzymes such as stomach acid and pancreatic enzyme, and is not easy to be degraded.

What is the difference between Apip and other similar molecules?

The Apip differs from other similar molecules in that it is a highly stable protein that can remain intact under extreme conditions.

What is the safety of Apip in clinical trials?

Clinical trials of Apip have shown a high safety profile without any serious adverse reactions.

What is the safety of Apip ?

The safety of Apip is very high, and no adverse effects on the body have been found in animal experiments.

What are the application areas of Apip ?

Apip can be used in the production of antitoxins, vaccines and other fields.

What are the characteristics of Apip s compared with other s?

Apip s are characterized by being more stable in high temperature and acid-base environments, which makes them more advantageous in some special application scenarios.

What is the effect of Apip in animal experiments?

Recombinant Apip protein can significantly increase platelet count and shorten bleeding time and hemostasis time in animal experiments.

What is the production cost of Apip ?

The production cost of Apip varies according to different factors such as production scale and process, and is generally at a moderate level.

How toxic is Apip ?

The Apip is a very safe protein with low toxicity.

What is the role of Apip in medical research?

Apip can be used to cause diseases such as asthma, influenza, bronchitis and other models for the study of drugs to treat various related diseases.

What are the biological characteristics of Apip ?

The Apip is a very stable protein that can remain intact under extreme conditions such as acid-base environments and high temperatures.