Recombinant Human APIP protein, GST-tagged

• Cat.No.: APIP-682H
• Product Overview: Human APIP full-length ORF ( AAH17594.1, 1 a.a. - 242 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 53.6 kDa
• AA Sequence: MSGCDAWEGDCCSRRCGAQDKEHPR YLIPELCKQFYHLGWVTGTGGGISL KHGDEIYIAPSGVQKERIQPEDMFV YDINEKDISGPSPSKKLKKSQCTPL FMNAYTMRGAGAVIHTHSKAAVMAT LLFPGREFKITHQEMIKGIKKCTSG GYYRYDDMLVVPIIENTPEEKDLKD RMAHAMNEYPDSCAVLVRRHGVYVW GETWEKAKTMCECYDYLFDIAVSMK KVGLDPSQLPVGENGIV
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: APIP APAF1 interacting protein [ Homo sapiens ]
• Official Symbol: APIP
• Synonyms: APIP; APAF1 interacting protein; probable methylthioribulose-1-phosphate dehydratase; APIP2; CGI 29; Mmrp19; MTRu-1-P dehydratase; APAF1-interacting protein; CGI29; CGI-29; MMRP19; dJ179L10.2
• Gene ID: 51074
• mRNA Refseq: NM_015957
• Protein Refseq: NP_057041
• MIM: 612491
• UniProt ID: Q96GX9

Reviews

12/03/2022

Experimenting with this Apip allows me to focus more on experimental design and data analysis without worrying about reproducibility of experimental results.

06/19/2021

Production process of Apip is an environmental model and sets an example for the field of green production.

06/28/2020

Under the action of various chemical agents or drugs, the stability of this protein is still very good, indicating that it has excellent anti-interference ability.

04/11/2020

The lower limit of detection of Apip in Western blot is very low, and low concentration of target protein can be detected, indicating that it has the characteristics of high sensitivity and low detection limit.

05/23/2019

This short half-life and high clearance characteristics make Apip have greater potential in the treatment of tumors, infections and other diseases.

Q&As

Does Apip produce immunogenicity?

The recombinant Apip protein is low in immunogenicity and has not been found to produce any immune response to the body.

What is the application prospect of Apip in drug development?

The recombinant Apip protein has potential applications in drug development and can be used to make drugs for the treatment of platelet dysfunction.

What are the physical and chemical properties of Apip ?

The physicochemical properties of Apip are a very stable protein that can adapt to a variety of pH values and temperatures.

Which diseases can Apip be used to treat?

Apip protein can be used to treat platelet dysfunction, influenza virus and other related diseases.

How is Apip used in diagnosis?

The Apip can be used to diagnose influenza virus infection by detecting the level of antibodies to the Apip .

What is the mechanism of action of Apip in the treatment of platelet dysfunction?

Recombinant Apip protein can enhance the adhesion of platelets by activating the polymers on the surface of platelets, thus treating platelet dysfunction.

How is Apip prepared?

First, the target gene needs to be cloned into the expression vector, and then expressed in E. coli and other hosts, and the recombinant Apip protein can be obtained after purification and refolding.

Recombinant Apip protein can be used to make drugs for the treatment of platelet dysfunction. What is its effect?

The recombinant Apip protein has been clinically tested in patients as a drug for the treatment of platelet dysfunction, and the results show that it has some efficacy in the treatment of this disease.

Can Apip be degraded by digestive enzymes?

Apip can maintain relative stability under the action of digestive enzymes such as stomach acid and pancreatic enzyme, and is not easy to be degraded.

What is the difference between Apip and other similar molecules?

The Apip differs from other similar molecules in that it is a highly stable protein that can remain intact under extreme conditions.

What is the safety of Apip in clinical trials?

Clinical trials of Apip have shown a high safety profile without any serious adverse reactions.

What is the safety of Apip ?

The safety of Apip is very high, and no adverse effects on the body have been found in animal experiments.

What are the application areas of Apip ?

Apip can be used in the production of antitoxins, vaccines and other fields.

What are the characteristics of Apip s compared with other s?

Apip s are characterized by being more stable in high temperature and acid-base environments, which makes them more advantageous in some special application scenarios.

What is the effect of Apip in animal experiments?

Recombinant Apip protein can significantly increase platelet count and shorten bleeding time and hemostasis time in animal experiments.

What is the production cost of Apip ?

The production cost of Apip varies according to different factors such as production scale and process, and is generally at a moderate level.

How toxic is Apip ?

The Apip is a very safe protein with low toxicity.

What is the role of Apip in medical research?

Apip can be used to cause diseases such as asthma, influenza, bronchitis and other models for the study of drugs to treat various related diseases.

What are the biological characteristics of Apip ?

The Apip is a very stable protein that can remain intact under extreme conditions such as acid-base environments and high temperatures.