Recombinant Human ARFRP1 protein, GST-tagged

• Cat.No.: ARFRP1-755H
• Product Overview: Human ARFRP1 full-length ORF ( NP_003215, 1 a.a. - 201 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2012]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 47.74 kDa
• AA Sequence: MYTLLSGLYKYMFQKDEYCILILGL DNAGKTTFLEQSKTRFNKNYKGMSL SKITTTVGLNIGTVDVGKARLMFWD LGGQEELQSLWDKYYAECHGVIYVI DSTDEERLAESKQAFEKVVTSEALC GVPVLVLANKQDVETCLSIPDIKTA FSDCTSKIGRRDCLTQACSALTGKG VREGIEWMVKCVVRNVHRPPRQRDI T
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARFRP1 ADP-ribosylation factor related protein 1 [ Homo sapiens ]
• Official Symbol: ARFRP1
• Synonyms: ARFRP1; ADP-ribosylation factor related protein 1; ADP-ribosylation factor-related protein 1; ARL18; ARP; Arp1; SCG10 like-protein; ARF-related protein 1; helicase-like protein NHL
• Gene ID: 10139
• mRNA Refseq: NM_001134758
• Protein Refseq: NP_001128230
• MIM: 604699
• UniProt ID: Q13795

Reviews

12/18/2022

The outstanding level of customer service adds extra reassurance and confidence in using the ARFRP1 protein for my scientific investigations.

10/24/2020

the manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of ARFRP1 protein in trials.

09/27/2020

manufacturers often have a wealth of knowledge and expertise in the field of ARFRP1 protein research.

12/09/2019

Collaborative discussions with the manufacturer can also help optimize experimental protocols and provide insights into the best practices in working with ARFRP1.

11/09/2016

The ARFRP1 protein offers numerous advantages in trials and research studies, making it an excellent choice for scientists in need of reliable and high-quality experimental materials.

10/18/2016

Its unique properties make ARFRP1 an invaluable tool for investigating cellular signaling pathways, protein-protein interactions, and membrane dynamics.

02/02/2016

With the ARFRP1 protein and its accompanying technical assistance, I am confident that I can achieve successful outcomes for my research endeavors.

01/02/2016

They can offer specialized formulations, concentrations, or modifications of ARFRP1 protein to suit unique experimental requirements.

Q&As

Are there any disease associations linked to ARFRP1?

Currently, there are no known disease associations directly linked to ARFRP1. However, the dysregulation of ARF family members, including ARFRP1, has been implicated in various diseases such as cancer and neurodegenerative disorders. Further research is needed to fully understand the role of ARFRP1 in these conditions.

Are there any known mutations or genetic variations in the ARFRP1 gene?

To date, there have been no reported disease-causing mutations or significant genetic variations identified in the ARFRP1 gene. However, genetic studies investigating potential associations between ARFRP1 and certain diseases are ongoing.

Is ARFRP1 involved in any other cellular processes?

While its primary role appears to be in vesicle trafficking, ARFRP1 has also been implicated in other cellular processes. It has been associated with actin cytoskeleton organization, cell migration, and modulation of cell surface receptors. However, further research is needed to fully understand its involvement in these processes.

Can ARFRP1 be targeted for therapeutic purposes?

The potential therapeutic targeting of ARFRP1 remains largely unexplored due to limited knowledge about its precise functions and mechanisms. However, as our understanding of ARFRP1 grows, it may present opportunities for the development of drugs or therapeutic approaches that modulate its activity, particularly in diseases associated with aberrant membrane trafficking processes.

How is ARFRP1 involved in vesicle trafficking?

ARFRP1 participates in vesicle trafficking by regulating the assembly and disassembly of coat proteins on transport vesicles. It is involved in the formation of COPI-coated vesicles, which mediate retrograde transport from the Golgi apparatus back to the ER. ARFRP1 interacts with ARFGAP1, an ARF GTPase-activating protein, to regulate COPI coat assembly and vesicle budding.

Are there any animal models available for studying ARFRP1?

Currently, there are no specific animal models developed solely for studying ARFRP1. However, studies have explored the effects of ARFRP1 depletion or overexpression in cellular and animal models, shedding light on its functional significance in various biological processes.

Can ARFRP1 be targeted for cancer therapy?

While there is no direct evidence for targeting ARFRP1 in cancer therapy, aberrant membrane trafficking is a hallmark of cancer progression. Given ARFRP1's involvement in vesicle trafficking and its potential role in tumor development, it may be worth investigating its therapeutic potential in cancer treatment or as a target for drug development in combination with other therapies.

Does ARFRP1 have any known interacting proteins?

ARFRP1 has been found to interact with various proteins involved in membrane trafficking and vesicle formation. Some of its known interacting partners include COPI (coatomer protein complex I) subunits and proteins associated with clathrin-mediated endocytosis.

Are there any known ARFRP1 knockout or knockdown models?

Yes, knockout and knockdown models of ARFRP1 have been generated in several organisms for research purposes. These models include genetically modified mice lacking ARFRP1 expression (ARFRP1 knockout mice) and cellular models in which ARFRP1 expression is specifically reduced or silenced (ARFRP1 knockdown models). Using these models, researchers have been able to study the function of ARFRP1 and its role in different cellular processes.

Does ARFRP1 have any post-translational modifications?

Yes, ARFRP1 can undergo post-translational modifications, including lipidation and phosphorylation. Lipid modification, such as lipid anchor addition, is crucial for ARFRP1's association with membranes. Phosphorylation events may regulate its activity, localization, or interactions with binding partners, although specific details regarding phosphorylation sites and functional consequences are still being explored.