Recombinant Mouse Arfrp1 Protein, Myc/DDK-tagged
Cat.No. : | Arfrp1-1677M |
Product Overview : | Purified recombinant protein of mouse full-length ADP-ribosylation factor related protein 1 (cDNA clone MGC:61235 IMAGE:5720540), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Description : | The gene encodes a membrane-associated GTPase that is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) genes. It plays an essential role in Golgi function controlling recruitment of GRIP domain proteins and ARL1 to the trans-Golgi and trans-Golgi to plasma membrane trafficking of cell surface proteins such as E-cadherin. Deletion of this gene in mice leads to embryonic lethality during early gastrulation, which is at least partly caused by the disruption of E-cadherin trafficking to the cell surface and therefore lack of sufficient cell-cell adhesion in the embryo. Multiple transcript variants encoding different isoforms have been found for this gene. |
Source : | HEK293T |
Species : | Mouse |
Tag : | Myc/DDK |
Molecular Mass : | 13.9 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name : | Arfrp1 ADP-ribosylation factor related protein 1 [ Mus musculus (house mouse) ] |
Official Symbol : | Arfrp1 |
Synonyms : | Arfrp1; ADP-ribosylation factor related protein 1; AI480700; 1500006I01Rik; ADP-ribosylation factor-related protein 1; ARF-related protein 1; EC 3.6.5.1; EC 3.6.5.2; EC 3.6.5.3; EC 3.6.5.4 |
Gene ID : | 76688 |
mRNA Refseq : | NM_029702 |
Protein Refseq : | NP_083978 |
UniProt ID : | Q8BXL7 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCurrently, there are no known disease associations directly linked to ARFRP1. However, the dysregulation of ARF family members, including ARFRP1, has been implicated in various diseases such as cancer and neurodegenerative disorders. Further research is needed to fully understand the role of ARFRP1 in these conditions.
To date, there have been no reported disease-causing mutations or significant genetic variations identified in the ARFRP1 gene. However, genetic studies investigating potential associations between ARFRP1 and certain diseases are ongoing.
While its primary role appears to be in vesicle trafficking, ARFRP1 has also been implicated in other cellular processes. It has been associated with actin cytoskeleton organization, cell migration, and modulation of cell surface receptors. However, further research is needed to fully understand its involvement in these processes.
The potential therapeutic targeting of ARFRP1 remains largely unexplored due to limited knowledge about its precise functions and mechanisms. However, as our understanding of ARFRP1 grows, it may present opportunities for the development of drugs or therapeutic approaches that modulate its activity, particularly in diseases associated with aberrant membrane trafficking processes.
ARFRP1 participates in vesicle trafficking by regulating the assembly and disassembly of coat proteins on transport vesicles. It is involved in the formation of COPI-coated vesicles, which mediate retrograde transport from the Golgi apparatus back to the ER. ARFRP1 interacts with ARFGAP1, an ARF GTPase-activating protein, to regulate COPI coat assembly and vesicle budding.
Currently, there are no specific animal models developed solely for studying ARFRP1. However, studies have explored the effects of ARFRP1 depletion or overexpression in cellular and animal models, shedding light on its functional significance in various biological processes.
While there is no direct evidence for targeting ARFRP1 in cancer therapy, aberrant membrane trafficking is a hallmark of cancer progression. Given ARFRP1's involvement in vesicle trafficking and its potential role in tumor development, it may be worth investigating its therapeutic potential in cancer treatment or as a target for drug development in combination with other therapies.
ARFRP1 has been found to interact with various proteins involved in membrane trafficking and vesicle formation. Some of its known interacting partners include COPI (coatomer protein complex I) subunits and proteins associated with clathrin-mediated endocytosis.
Yes, knockout and knockdown models of ARFRP1 have been generated in several organisms for research purposes. These models include genetically modified mice lacking ARFRP1 expression (ARFRP1 knockout mice) and cellular models in which ARFRP1 expression is specifically reduced or silenced (ARFRP1 knockdown models). Using these models, researchers have been able to study the function of ARFRP1 and its role in different cellular processes.
Yes, ARFRP1 can undergo post-translational modifications, including lipidation and phosphorylation. Lipid modification, such as lipid anchor addition, is crucial for ARFRP1's association with membranes. Phosphorylation events may regulate its activity, localization, or interactions with binding partners, although specific details regarding phosphorylation sites and functional consequences are still being explored.
Customer Reviews (8)
Write a reviewThe outstanding level of customer service adds extra reassurance and confidence in using the ARFRP1 protein for my scientific investigations.
the manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of ARFRP1 protein in trials.
manufacturers often have a wealth of knowledge and expertise in the field of ARFRP1 protein research.
Collaborative discussions with the manufacturer can also help optimize experimental protocols and provide insights into the best practices in working with ARFRP1.
The ARFRP1 protein offers numerous advantages in trials and research studies, making it an excellent choice for scientists in need of reliable and high-quality experimental materials.
Its unique properties make ARFRP1 an invaluable tool for investigating cellular signaling pathways, protein-protein interactions, and membrane dynamics.
With the ARFRP1 protein and its accompanying technical assistance, I am confident that I can achieve successful outcomes for my research endeavors.
They can offer specialized formulations, concentrations, or modifications of ARFRP1 protein to suit unique experimental requirements.
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