Recombinant Human ARHGAP27 293 Cell Lysate

• Cat.No.: ARHGAP27-8740HCL

Specification

• Description: Antigen standard for Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARHGAP27 Rho GTPase activating protein 27 [ Homo sapiens ]
• Official Symbol: ARHGAP27
• Synonyms: ARHGAP27; Rho GTPase activating protein 27; SH3 domain containing 20 , SH3D20; rho GTPase-activating protein 27; CAMGAP1; FLJ43547; SH3P20; SH3 domain containing 20; SH3 domain-containing protein 20; rho-type GTPase-activating protein 27; CIN85-associated multi-domain-containing Rho GTPase-activating protein 1; PP905; SH3D20; FLJ35572; FLJ76025; MGC78393; MGC120624
• Gene ID: 201176
• mRNA Refseq: NM_174919
• Protein Refseq: NP_777579
• MIM: 610591
• UniProt ID: Q6ZUM4
• Chromosome Location: 17q21.31
• Function: GTPase activator activity; Rac GTPase activator activity; SH3 domain binding

Reviews

04/26/2023

Their team of experts is readily available to address any queries or concerns I may have regarding the ARHGAP27 protein.

08/14/2022

Their team of experts is readily available to address any concerns or queries I may have regarding the ARHGAP27 protein.

06/18/2021

when employed in Western Blotting experiments, the ARHGAP27 protein consistently generates sharp and well-defined protein bands, enabling precise visualization and analysis of protein expression.

12/03/2017

I highly recommend the use of the ARHGAP27 protein in various experimental applications.

10/06/2017

the manufacturer's supply management capabilities assure a seamless and continuous provision of the ARHGAP27 protein.

05/10/2017

The ARHGAP27 protein offered by the manufacturer excels in its quality and is an excellent match for my experimental requirements.

11/16/2016

the ARHGAP27 protein has been successfully utilized in protein electron microscopy structure analysis, providing valuable insights into molecular structures and interactions.

06/06/2016

Considering its outstanding performance across multiple assays, I confidently endorse the inclusion of the ARHGAP27 protein in diverse research studies.

Q&As

Does ARHGAP27 have any known protein isoforms?

Currently, there are no known protein isoforms of ARHGAP27. However, alternative splicing events may give rise to potential isoforms that have not yet been characterized.

Are there any animal models available to study ARHGAP27 function?

As of now, there are no specific animal models available that solely focus on ARHGAP27. However, mouse models with altered Rho GTPase signaling or cytoskeletal dynamics could potentially help in studying ARHGAP27's function indirectly.

Does ARHGAP27 have any known roles in immune system function?

At present, there is limited information on the involvement of ARHGAP27 in immune system function. Further research is necessary to determine if ARHGAP27 plays a role in immune responses or immune cell function.

Is ARHGAP27 expressed in all tissues or is it tissue-specific?

ARHGAP27 has been found to be widely expressed across different tissues in the body. It has been detected in various cell types, including neurons, endothelial cells, fibroblasts, and immune cells.

Is there any evidence suggesting a role for ARHGAP27 in cancer progression?

Limited research has been done on the role of ARHGAP27 in cancer. However, some studies have shown altered expression of ARHGAP27 in certain cancer types, suggesting its potential involvement in cancer development and progression. Further investigation is needed to fully understand its role in cancer biology.

Is ARHGAP27 involved in neuronal development or synaptic function?

The specific role of ARHGAP27 in neuronal development or synaptic function is not well characterized. More research is needed to determine its potential involvement in these processes.

Can ARHGAP27 expression be regulated by external factors or stimuli?

Currently, there is limited information on the regulation of ARHGAP27 expression by external factors or stimuli. Further studies are required to determine if its expression can be influenced by specific cellular signaling pathways or environmental cues.

Are there any known genetic disorders associated with mutations in the ARHGAP27 gene?

Currently, there are no known genetic disorders specifically linked to mutations in the ARHGAP27 gene.

Does ARHGAP27 play any role in cell division or cell proliferation?

The involvement of ARHGAP27 in cell division or cell proliferation is not well established. While it has been implicated in regulating cytoskeletal dynamics, more research is needed to determine if it plays a direct role in these processes.

Does ARHGAP27 have any known functional domains?

ARHGAP27 contains a RhoGAP domain, which suggests its involvement in regulating the activity of Rho GTPases. Additionally, it has a coiled-coil domain, which may be important for protein-protein interactions.

Are there any studies linking ARHGAP27 to neurological disorders or neurodegeneration?

Currently, there are no specific studies directly linking ARHGAP27 to neurological disorders or neurodegeneration. However, given its role in cytoskeletal dynamics and potential involvement in neuronal development, further research may shed light on its relevance in these conditions.

Can ARHGAP27 be targeted for therapeutic purposes?

Currently, there is no known therapeutic targeting of ARHGAP27. However, further research into its function and involvement in diseases may suggest potential therapeutic strategies in the future.

Can ARHGAP27 interact with other proteins?

Yes, ARHGAP27 has been found to interact with various proteins involved in cell signaling and cytoskeletal organization. Some of its known binding partners include Rho GTPases, such as RhoA and Cdc42, as well as proteins involved in actin nucleation and polymerization.

Are there any known cellular processes that ARHGAP27 regulates?

ARHGAP27 is believed to play a role in regulating actin cytoskeleton dynamics and cell migration, based on its interaction with Rho GTPases. However, the exact cellular processes it regulates are still being investigated.