Recombinant Mouse Arhgap27 Protein, Myc/DDK-tagged

• Cat.No.: Arhgap27-1685M
• Product Overview: Purified recombinant protein of mouse full-length Rho GTPase activating protein 27 (Arhgap27), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 75.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Arhgap27 Rho GTPase activating protein 27 [ Mus musculus (house mouse) ]
• Official Symbol: Arhgap27
• Synonyms: Arhgap27; Rho GTPase activating protein 27; Sh3d2; Sh3d20; Gm21552; 2310069I04Rik; 5730442P18Rik; rho GTPase-activating protein 27; CIN85-associated multi-domain-containing Rho GTPase-activating protein 1; SH3 domain containing 20; rho-type GTPase-activating protein 27
• Gene ID: 544817
• mRNA Refseq: NM_133715
• Protein Refseq: NP_598476
• UniProt ID: A2AB59

Reviews

04/26/2023

Their team of experts is readily available to address any queries or concerns I may have regarding the ARHGAP27 protein.

08/14/2022

Their team of experts is readily available to address any concerns or queries I may have regarding the ARHGAP27 protein.

06/18/2021

when employed in Western Blotting experiments, the ARHGAP27 protein consistently generates sharp and well-defined protein bands, enabling precise visualization and analysis of protein expression.

12/03/2017

I highly recommend the use of the ARHGAP27 protein in various experimental applications.

10/06/2017

the manufacturer's supply management capabilities assure a seamless and continuous provision of the ARHGAP27 protein.

05/10/2017

The ARHGAP27 protein offered by the manufacturer excels in its quality and is an excellent match for my experimental requirements.

11/16/2016

the ARHGAP27 protein has been successfully utilized in protein electron microscopy structure analysis, providing valuable insights into molecular structures and interactions.

06/06/2016

Considering its outstanding performance across multiple assays, I confidently endorse the inclusion of the ARHGAP27 protein in diverse research studies.

Q&As

Does ARHGAP27 have any known protein isoforms?

Currently, there are no known protein isoforms of ARHGAP27. However, alternative splicing events may give rise to potential isoforms that have not yet been characterized.

Are there any animal models available to study ARHGAP27 function?

As of now, there are no specific animal models available that solely focus on ARHGAP27. However, mouse models with altered Rho GTPase signaling or cytoskeletal dynamics could potentially help in studying ARHGAP27's function indirectly.

Does ARHGAP27 have any known roles in immune system function?

At present, there is limited information on the involvement of ARHGAP27 in immune system function. Further research is necessary to determine if ARHGAP27 plays a role in immune responses or immune cell function.

Is ARHGAP27 expressed in all tissues or is it tissue-specific?

ARHGAP27 has been found to be widely expressed across different tissues in the body. It has been detected in various cell types, including neurons, endothelial cells, fibroblasts, and immune cells.

Is there any evidence suggesting a role for ARHGAP27 in cancer progression?

Limited research has been done on the role of ARHGAP27 in cancer. However, some studies have shown altered expression of ARHGAP27 in certain cancer types, suggesting its potential involvement in cancer development and progression. Further investigation is needed to fully understand its role in cancer biology.

Is ARHGAP27 involved in neuronal development or synaptic function?

The specific role of ARHGAP27 in neuronal development or synaptic function is not well characterized. More research is needed to determine its potential involvement in these processes.

Can ARHGAP27 expression be regulated by external factors or stimuli?

Currently, there is limited information on the regulation of ARHGAP27 expression by external factors or stimuli. Further studies are required to determine if its expression can be influenced by specific cellular signaling pathways or environmental cues.

Are there any known genetic disorders associated with mutations in the ARHGAP27 gene?

Currently, there are no known genetic disorders specifically linked to mutations in the ARHGAP27 gene.

Does ARHGAP27 play any role in cell division or cell proliferation?

The involvement of ARHGAP27 in cell division or cell proliferation is not well established. While it has been implicated in regulating cytoskeletal dynamics, more research is needed to determine if it plays a direct role in these processes.

Does ARHGAP27 have any known functional domains?

ARHGAP27 contains a RhoGAP domain, which suggests its involvement in regulating the activity of Rho GTPases. Additionally, it has a coiled-coil domain, which may be important for protein-protein interactions.

Are there any studies linking ARHGAP27 to neurological disorders or neurodegeneration?

Currently, there are no specific studies directly linking ARHGAP27 to neurological disorders or neurodegeneration. However, given its role in cytoskeletal dynamics and potential involvement in neuronal development, further research may shed light on its relevance in these conditions.

Can ARHGAP27 be targeted for therapeutic purposes?

Currently, there is no known therapeutic targeting of ARHGAP27. However, further research into its function and involvement in diseases may suggest potential therapeutic strategies in the future.

Can ARHGAP27 interact with other proteins?

Yes, ARHGAP27 has been found to interact with various proteins involved in cell signaling and cytoskeletal organization. Some of its known binding partners include Rho GTPases, such as RhoA and Cdc42, as well as proteins involved in actin nucleation and polymerization.

Are there any known cellular processes that ARHGAP27 regulates?

ARHGAP27 is believed to play a role in regulating actin cytoskeleton dynamics and cell migration, based on its interaction with Rho GTPases. However, the exact cellular processes it regulates are still being investigated.