Recombinant Human ARHGEF18 Protein (159-551 aa), GST-tagged

• Cat.No.: ARHGEF18-1214H
• Product Overview: Recombinant Human ARHGEF18 Protein (159-551 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Research Area: Signal Transduction. Protein Description: Partial.

Specification

• Description: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. Also act as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 71.5 kDa
• Protein length: 159-551 aa
• AA Sequence: MTVSQKGGPQPTPSPAGPGTQLGPI TGEMDEADSAFLKFKQTADDSLSLT SPNTESIFVEDPYTASLRSEIESDG HEFEAESWSLAVDAAYAKKQKREVV KRQDVLYELMQTEVHHVRTLKIMLK VYSRALQEELQFSSKAIGRLFPCAD DLLETHSHFLARLKERRQESLEEGS DRNYVIQKIGDLLVQQFSGENGERM KEKYGVFCSGHNEAVSHYKLLLQQN KKFQNLIKKIGNFSIVRRLGVQECI LLVTQRITKYPVLVERIIQNTEAGT EDYEDLTQALNLIKDIISQVDAKVS ECEKGQRLREIAGKMDLKSSSKLKN GLTFRKEDMLQRQLHLEGMLCWKTT SGRLKDILAILLTDVLLLLQEKDQK YVFASVDSKPPVISLQKL
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: ARHGEF18 Rho/Rac guanine nucleotide exchange factor (GEF) 18 [ Homo sapiens ]
• Official Symbol: ARHGEF18
• Synonyms: ARHGEF18; KIAA0521; MGC15913; P114 RhoGEF; SA-RhoGEF; p114RhoGEF; P114-RHO-GEF; P114-RhoGEF
• Gene ID: 23370
• mRNA Refseq: NM_001130955
• Protein Refseq: NP_001124427
• UniProt ID: Q6ZSZ5

Reviews

03/17/2023

The manufacturer ensures that the protein is produced with utmost purity and integrity, minimizing the risk of experimental artifacts or compromised results.

04/30/2022

With the ARHGEF18 protein as a research tool, achieving meaningful scientific findings becomes an accessible and achievable goal.

12/20/2021

The ARHGEF18 protein is a superior-quality protein that perfectly aligns with my experimental requirements.

11/15/2021

This high-quality ARHGEF18 protein provides researchers with confidence in obtaining accurate and reliable data during their investigations

01/07/2020

Their expertise and dedication enable them to effectively address and resolve any technical issues that may arise during experimentation.

09/10/2019

The manufacturer's team of experts possesses a deep understanding of the ARHGEF18 protein and its associated protocols.

09/17/2018

the ARHGEF18 protein's versatility extends beyond its use in vitro experiments.

07/04/2018

In conclusion, using ARHGEF18 protein in trials offers distinct advantages for researchers studying cell cycle regulation.

Q&As

Are there any genetic mutations in ARHGEF18 associated with human diseases?

Yes, mutations in the ARHGEF18 gene have been identified in individuals with neurodevelopmental disorders. These mutations are thought to disrupt the normal function of ARHGEF18 and contribute to cognitive impairments, intellectual disabilities, and developmental delays.

Is ARHGEF18 involved in any other cellular processes?

Beyond its roles in cancer and neuronal function, ARHGEF18 has also been implicated in other cellular processes. It contributes to the regulation of cell proliferation, cell adhesion, and cytoskeletal dynamics. Additionally, it may play a role in immune responses, as some studies have reported its involvement in the activation and migration of immune cells.

Are there any known diseases or disorders associated with ARHGEF18?

Yes, emerging evidence suggests that ARHGEF18 may be involved in the pathogenesis of certain diseases and disorders. Variants in ARHGEF18 have been implicated in neurodevelopmental disorders such as autism spectrum disorder and intellectual disability. Additionally, altered expression of ARHGEF18 has been linked to cancer progression and metastasis in various types of malignancies.

Can genetic variations in ARHGEF18 affect an individual's susceptibility to diseases?

There is limited research on the role of genetic variations in ARHGEF18 and disease susceptibility. However, some studies have identified specific genetic variants in ARHGEF18 that may contribute to the risk or severity of certain conditions, including neurodevelopmental disorders and certain types of cancer.

Can you provide an overview of the signaling pathways regulated by ARHGEF18?

ARHGEF18 primarily regulates the signaling pathways associated with Rho GTPases, such as RhoA, Rac1, and Cdc42. Activation of ARHGEF18 leads to the exchange of GDP for GTP on these GTPases, subsequently activating them and initiating downstream signaling cascades. These pathways play significant roles in cytoskeletal rearrangements, cell migration, cell adhesion, and synaptic plasticity.

Is ARHGEF18 protein involved in any specific signaling pathways?

ARHGEF18 is primarily associated with the Rho signaling pathway. As a guanine nucleotide exchange factor, it activates Rho GTPases, which in turn regulate downstream signaling cascades involved in various cellular processes. These include pathways associated with cell adhesion, migration, proliferation, and cytoskeletal dynamics.

Does ARHGEF18 play a role in neuronal development or function?

Yes, ARHGEF18 is known to be involved in neuronal development and synaptic plasticity. It is expressed in the brain and contributes to the formation and maintenance of neuronal connections. Dysregulation of ARHGEF18 has been linked to neurodevelopmental disorders and neurodegenerative diseases.

Are there any known inhibitors or activators of ARHGEF18?

There are currently no specific inhibitors or activators designed specifically for ARHGEF18. However, certain small molecules and compounds that target Rho GTPases, such as Rho kinase inhibitors or Rac1 activators, indirectly affect the activity of ARHGEF18 by modulating the downstream signaling pathways it participates in.

How is ARHGEF18 linked to neurodevelopmental disorders like autism spectrum disorder?

Genetic studies have identified rare variants in ARHGEF18 that are associated with an increased risk for autism spectrum disorder and intellectual disability. These variants may disrupt the normal function of ARHGEF18 in neuronal development and synaptic plasticity, leading to impairments in neural connectivity and associated behavioral abnormalities. Further research is ongoing to understand the specific molecular mechanisms through which ARHGEF18 contributes to these neurodevelopmental disorders.

Are there any interacting partners of ARHGEF18?

Yes, ARHGEF18 can interact with several proteins to form protein complexes that regulate its function. Some known interacting partners include Rho GTPases (such as RhoA, Rac1, and Cdc42), other guanine nucleotide exchange factors, and cytoskeletal proteins involved in actin dynamics.

Are there any known interactions between ARHGEF18 and other proteins?

Yes, ARHGEF18 can interact with several proteins to mediate its cellular functions. These interactions may involve its binding to other guanine nucleotide exchange factors, Rho GTPases, or proteins involved in cytoskeletal dynamics and cell signaling. Some of the known interacting partners of ARHGEF18 include α-actinin, filamin, IQGAP1, and paxillin.

Are there any therapeutic implications regarding ARHGEF18?

Given its involvement in cancer and neurodevelopmental disorders, ARHGEF18 holds potential as a therapeutic target. Identifying small molecules or approaches to modulate ARHGEF18 activity could potentially be explored to inhibit tumor metastasis or ameliorate symptoms associated with neurodevelopmental disorders. However, further research is needed to better understand the precise mechanisms of ARHGEF18 and its potential therapeutic applications.

What research advancements have been made regarding ARHGEF18 in recent years?

Recent research on ARHGEF18 has focused on its role in neurodevelopmental disorders and cancer. Studies have identified genetic variants in ARHGEF18 associated with increased risk for autism spectrum disorder and intellectual disability. In cancer research, ARHGEF18 has been implicated in tumor metastasis and invasiveness. Additionally, the functional mechanisms and interacting partners of ARHGEF18 are continuously being investigated to better understand its role in cellular processes and associated pathologies.

How is ARHGEF18 protein linked to cell migration and invasion in cancer?

ARHGEF18 promotes cell migration and invasion in cancer by activating Rho GTPases, particularly RhoA and Rac1, which regulate cytoskeletal rearrangements necessary for cell movement. ARHGEF18 expression is upregulated in cancer cells, leading to increased cell motility and invasiveness, which contribute to tumor metastasis.