Recombinant Human ARHGEF5 cell lysate

• Cat.No.: ARHGEF5-118HCL

Specification

• Description: Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.
• Species: Human
• Size: 100 ul
• Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
• Applications: Western Blot

Gene Information

• Gene Name: ARHGEF5 Rho guanine nucleotide exchange factor (GEF) 5 [ Homo sapiens ]
• Official Symbol: ARHGEF5
• Synonyms: P60; TIM; GEF5; TIM1; rho guanine nucleotide exchange factor 5; p60 TIM; ephexin-3; oncogene TIM; guanine nucleotide regulatory protein TIM; transforming immortalized mammary oncogene
• Gene ID: 7984
• mRNA Refseq: NM_005435
• Protein Refseq: NP_005426
• MIM: 600888
• UniProt ID: Q12774
• Chromosome Location: 7q35
• Pathway: Regulation of RhoA activity, organism-specific biosystem
• Function: GTP binding; Rho guanyl-nucleotide exchange factor activity; protein binding

Reviews

03/11/2023

Its versatility allows researchers to explore ARHGEF5's involvement in diverse biological processes and fully uncover its functional role.

07/30/2022

the manufacturer's technical expertise can be advantageous to researchers.

10/27/2020

In addition to the advantages of ARHGEF5 protein itself, the manufacturer can play a crucial role in supporting the research efforts.

04/16/2020

This quality control is essential for obtaining reliable and reproducible data, as any variability in protein quality can introduce confounding factors into experiments.

09/24/2019

the ARHGEF5 protein's versatility extends beyond its proven usefulness in in vitro experiments.

05/21/2019

the ARHGEF5 protein's high quality, dependable performance, and comprehensive technical support make it an excellent choice for researchers seeking to investigate angiopoietin-related biology and unravel the complexities of vascular development and pathogenesis.

09/06/2018

It can also be employed in various molecular biology techniques, such as Western blotting, immunoprecipitation, or protein-protein interaction studies.

12/18/2017

They can offer guidance on experimental design, protocol optimization, and troubleshooting, helping researchers overcome any challenges encountered during their trials.

Q&As

Is ARHGEF5 involved in neuronal development?

Yes, ARHGEF5 is involved in neuronal development and plays a role in neuronal morphology. It has been shown to influence neurite outgrowth, dendritic spine formation, and axonal guidance during brain development.

Are there any diseases associated with ARHGEF5 dysfunction?

Although limited, studies have suggested that dysregulation of ARHGEF5 may be linked to certain diseases. For instance, ARHGEF5 has been found to be upregulated in certain types of cancer, potentially contributing to tumor progression and metastasis. Further research is necessary to establish the specific role of ARHGEF5 in different pathological conditions.

In which cellular processes does ARHGEF5 play a role?

ARHGEF5 is involved in diverse cellular processes such as cell migration, adhesion, polarity, and proliferation. It regulates the organization of actin cytoskeleton and facilitates the formation of specific cellular structures like stress fibers and focal adhesions.

Is ARHGEF5 expression regulated during developmental stages?

ARHGEF5 expression can be dynamically regulated during development. Studies have shown that ARHGEF5 mRNA and protein levels vary depending on the developmental stage of specific tissues and organs. Additionally, various signaling pathways involved in developmental processes, such as Wnt and TGF-β, can influence ARHGEF5 expression.

Are there any known interacting partners of ARHGEF5?

Yes, ARHGEF5 has been found to interact with several proteins. One of its known interacting partners is Plexin-B1, a receptor involved in semaphorin signaling. ARHGEF5 also interacts with SRGAP1, which is involved in neuronal development and inhibits Rac1 activity. Additionally, ARHGEF5 has been shown to interact with several other Rho GEFs, such as ARHGEF6 and ARHGEF7, suggesting potential crosstalk between different Rho GTPase signaling pathways.

Are there any specific inhibitors or activators of ARHGEF5?

Currently, there are no specific inhibitors or activators designed specifically for ARHGEF5. However, small molecule inhibitors targeting Rho GTPase pathways, such as Rho kinase (ROCK) inhibitors, can indirectly affect the activity of ARHGEF5 and other Rho GEFs. Additionally, modulating upstream signaling pathways that regulate ARHGEF5, such as Wnt or TGF-β signaling, might indirectly influence its activity.

Can ARHGEF5 be targeted for drug development?

Targeting ARHGEF5 could be a potential strategy for drug development, specifically in diseases where Rho GTPase dysregulation is implicated. However, further research is needed to understand the precise role and regulation of ARHGEF5 in different pathological conditions before developing specific drugs against this protein.

Can ARHGEF5 be regulated by post-translational modifications?

Yes, ARHGEF5 can be regulated by post-translational modifications. Phosphorylation of specific residues within ARHGEF5 has been shown to modulate its activity. Other post-translational modifications, such as ubiquitination, acetylation, and SUMOylation, might also play a role in regulating ARHGEF5 function, although further research is needed to fully elucidate the extent of these modifications.

Are there any animal models available to study ARHGEF5 function?

Yes, animal models have been utilized to study the function of ARHGEF5. For instance, knockout mice lacking ARHGEF5 have been generated. These animal models allow researchers to investigate the role of ARHGEF5 in vivo and understand its contribution to development, disease, and cellular processes.

How does ARHGEF5 contribute to cell migration?

ARHGEF5 enhances cell migration by activating Rho GTPases, particularly RhoA and Rac1, which are crucial regulators of actin dynamics and cell motility. By activating these GTPases, ARHGEF5 promotes the reorganization of actin filaments and the formation of lamellipodia and filopodia necessary for cell migration.

What are the downstream effectors of ARHGEF5 signaling?

The downstream effectors of ARHGEF5 signaling are mainly the Rho GTPases it activates, such as RhoA and Rac1. These GTPases regulate a wide range of effector proteins, including various kinases and actin-binding proteins, ultimately impacting cellular processes like actin cytoskeleton rearrangement, cell adhesion, and cell migration.

How is ARHGEF5 expression regulated in cancer cells?

The regulation of ARHGEF5 expression in cancer cells is not fully understood. However, certain factors and signaling pathways associated with cancer progression, such as hypoxia-inducible factor-1 (HIF-1) and TGF-β, have been implicated in promoting ARHGEF5 expression. Additionally, genetic alterations, epigenetic modifications, and aberrant signaling pathways in cancer cells may contribute to dysregulated ARHGEF5 expression.

Can ARHGEF5 mutations lead to genetic disorders?

While there is no conclusive evidence of ARHGEF5 mutations causing a specific genetic disorder, genetic variations in ARHGEF5 have been associated with certain diseases. For example, a single nucleotide polymorphism (SNP) in ARHGEF5 has been linked to an increased risk of developing hypertension.

Does ARHGEF5 have any role in cancer progression?

There is evidence suggesting that ARHGEF5 may contribute to cancer progression. Elevated expression of ARHGEF5 has been observed in various cancer types, including breast, lung, and pancreatic cancer. ARHGEF5 promotes cancer cell migration, invasion, and metastasis, potentially making it a relevant target for therapeutic interventions.

Does ARHGEF5 have any homologs or related proteins?

Yes, ARHGEF5 belongs to a family of proteins called Rho guanine nucleotide exchange factors (Rho GEFs). This family includes other members such as ARHGEF1, ARHGEF7, and ARHGEF10, which also act as GEFs for Rho GTPases and play roles in various cellular processes.

Are there any specific cellular or molecular interactions of ARHGEF5?

ARHGEF5 has been reported to interact with various proteins and molecules. For example, it can interact with specific Rho GTPases, forming a complex that allows for the exchange of nucleotides. Additionally, ARHGEF5 may interact with other proteins involved in cytoskeletal organization and cell signaling, further contributing to its regulatory functions.