Recombinant Human ARL15 293 Cell Lysate

• Cat.No.: ARL15-8718HCL

Specification

• Description: Antigen standard for ADP-ribosylation factor-like 15 (ARL15) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARL15 ADP-ribosylation factor-like 15 [ Homo sapiens ]
• Official Symbol: ARL15
• Synonyms: ARL15; ADP-ribosylation factor-like 15; ADP ribosylation factor related protein 2 , ARFRP2; ADP-ribosylation factor-like protein 15; FLJ20051; ARF-related protein 2; ADP-ribosylation factor related protein 2; ADP-ribosylation factor-related protein 2; ARFRP2
• Gene ID: 54622
• mRNA Refseq: NM_019087
• Protein Refseq: NP_061960
• UniProt ID: Q9NXU5
• Chromosome Location: 5p15.2
• Function: GTP binding; nucleotide binding

Reviews

12/06/2022

Their technical expertise and experience in the field allow them to provide invaluable guidance and assistance.

09/30/2022

They have a deep understanding of the ARL15 protein and can provide valuable insights into its characteristics, properties, and optimal experimental conditions.

11/16/2020

the manufacturer offers outstanding technical support, serving as a valuable resource to resolve any issues that may arise during my research.

07/31/2020

This guidance can help researchers design and execute their experiments more effectively, ensuring reliable and accurate results.

06/14/2020

The manufacturer's support can play a crucial role in assisting researchers throughout their trials.

06/30/2018

They can offer insights on experimental design, optimal usage protocols, and troubleshoot any challenges that may arise during the course of the trial.

07/11/2017

In addition to these experimental benefits, the manufacturer of ARL15 protein plays a vital role in supporting researchers.

08/13/2016

Its potency and specificity make it a valuable tool for studying specific biological pathways or mechanisms.

Q&As

Can ARL15 be therapeutically targeted for any diseases?

Given its potential involvement in cancer development, ARL15 could be a potential therapeutic target for specific types of cancers. However, more studies are necessary to evaluate its suitability as a target for therapeutic intervention.

Is ARL15 conserved across different species?

ARL15 shows varying levels of conservation across species. While it is found in mammals, its presence in other organisms may differ. Comparative genomics studies can provide insights into the conservation of ARL15.

Has ARL15 been linked to any developmental processes?

There is limited knowledge about the involvement of ARL15 in developmental processes. Further research is required to explore its potential role in development and organogenesis.

Are there any genetic variants or mutations in the ARL15 gene associated with diseases?

Currently, there is limited information on specific genetic variants or mutations in the ARL15 gene associated with diseases. However, genome-wide association studies (GWAS) or genetic screenings could potentially provide insights into any disease-related variants.

Is ARL15 predominantly localized to a specific cellular compartment?

ARL15 is known to localize to different cellular compartments depending on the context. It has been observed in the cytoplasm, plasma membrane, and within vesicular structures involved in protein trafficking.

Are there any known interacting proteins or protein complexes that involve ARL15?

Yes, several proteins have been identified as interacting partners of ARL15. These include components of the cytoskeleton, such as actin, as well as proteins involved in vesicle transport and membrane trafficking pathways.

Has ARL15 been linked to any cellular processes or signaling pathways?

ARL15 has been associated with several cellular processes, including cell migration, cytoskeletal dynamics, and membrane trafficking. It may also play a role in downstream signaling pathways, although more research is needed to fully understand its involvement.

Are there any known functional studies or characterizations of ARL15?

While some functional studies have been conducted on ARL15, its exact role and mechanisms of action are still being explored. Further research is needed to fully understand its function.

Is ARL15 associated with any diseases or disorders?

There is evidence to suggest that ARL15 may be involved in certain diseases. For example, it has been implicated in the development of breast cancer and prostate cancer.