Recombinant Human ARL8A 293 Cell Lysate
Cat.No. : | ARL8A-8705HCL |
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Description : | Antigen standard for ADP-ribosylation factor-like 8A (ARL8A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ARL8A ADP-ribosylation factor-like 8A [ Homo sapiens ] |
Official Symbol : | ARL8A |
Synonyms : | ARL8A; ADP-ribosylation factor-like 8A; ADP ribosylation factor like 10B , ARL10B; ADP-ribosylation factor-like protein 8A; FLJ45195; Gie2; ADP-ribosylation factor-like 10B; novel small G protein indispensable for equal chromosome segregation 2; GIE2; ARL10B; |
Gene ID : | 127829 |
mRNA Refseq : | NM_001256129 |
Protein Refseq : | NP_001243058 |
UniProt ID : | Q96BM9 |
Chromosome Location : | 1q32.1 |
Function : | GTP binding; GTPase activity; alpha-tubulin binding; beta-tubulin binding; nucleotide binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (13)
Ask a questionYes, studies have shown that ARL8A can interact with other organelles such as the Golgi apparatus and mitochondria. This suggests that ARL8A may have additional functions in cellular processes related to these organelles.
ARL8A interacts with the microtubule plus-end-directed kinesin motor proteins to facilitate the transport of lysosomes along microtubules. This interaction helps position lysosomes near the periphery of cells, facilitating efficient fusion with phagosomes or other vesicles.
Dysregulation of lysosome function and trafficking pathways, including those involving ARL8A, has been implicated in neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Targeting ARL8A and related proteins could potentially provide therapeutic strategies to modulate lysosomal dysfunction and alleviate disease pathologies.
As of now, no genetic disorders or specific mutations in ARL8A have been identified. However, variations or alterations affecting the pathways regulated by ARL8A could potentially contribute to certain diseases or conditions.
ARL8A interacts with various proteins, including SKIP (SifA and kinesin-interacting protein) and VARP (VPS9-ankyrin repeat protein), which are involved in tethering and docking of phagosomes to lysosomes. Other known interacting partners include SKIPR-1, Hook1, and Dynactin.
Yes, ARL8A has been shown to play a role in autophagy, a cellular process responsible for the degradation of damaged organelles or proteins. It regulates the maturation and fusion of autophagosomes with lysosomes, aiding in the degradation of the autophagic cargo.
ARL8A is expressed in a variety of cell types, including both normal and cancer cells. Its expression levels may vary in different tissues and cell types, suggesting potential cell-specific functions or regulation.
Currently, there are no specific natural inhibitors or activators identified for ARL8A. However, studies are ongoing to discover small molecule compounds or modulators that can selectively target ARL8A activity for potential therapeutic applications.
Yes, ARL8A has been shown to be involved in immune responses by facilitating phagosome-lysosome fusion and degradation of engulfed pathogens in immune cells. Its interaction with proteins like SKIP and VARP in immune cells helps mediate the intracellular trafficking of phagosomes and regulate immune responses.
Currently, no specific diseases have been directly linked to ARL8A. However, dysregulation of lysosome-related processes, in which ARL8A is involved, can contribute to conditions such as lysosomal storage disorders and neurodegenerative diseases.
ARL8A has potential as a therapeutic target due to its involvement in various cellular processes and its role in diseases related to lysosomal dysfunction or membrane trafficking. However, more research is needed to fully understand its mechanisms and develop specific interventions.
Yes, ARL8A interacts with various proteins to carry out its cellular functions. It interacts with kinesin motor proteins, SKIP, and VARP, among others, to participate in organelle trafficking, immune responses, and phagosome-lysosome fusion.
Yes, ARL8A proteins are highly conserved across different species, including mammals, flies, worms, and plants. This suggests that ARL8A has important cellular functions that are preserved throughout evolution.
Customer Reviews (8)
Write a reviewIts reliable and accurate performance ensures dependable results, contributing to the advancement of scientific knowledge in various fields of study.
One of the key advantages of working with the ARL8A protein is the comprehensive support provided by its manufacturer.
Its robust performance has significantly accelerated my research progress and contributed to the advancement of my scientific understanding.
This information is crucial for researchers in understanding the protein's behavior and interaction within their specific experimental context.
Whether it's troubleshooting issues, optimizing experimental protocols, or providing detailed information about the protein, the manufacturer's support team is dedicated to helping researchers overcome any challenges they may encounter.
Their knowledgeable and responsive team assists in troubleshooting and finding solutions to any experimental challenges that may arise.
ARL8A is known for its high purity and quality, ensuring accurate and reproducible results.
This protein has become an invaluable asset in my research, offering great versatility in its applications.
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