Recombinant Human ARL8B protein, GST-tagged

• Cat.No.: ARL8B-301538H
• Product Overview: Recombinant Human ARL8B (1-186 aa) protein, fused to GST tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization
• Protein length: Met1-Ser186
• AA Sequence: MLALISRLLDWFRSLFWKEEMELTL VGLQYSGKTTFVNVIASGQFSEDMI PTVGFNMRKVTKGNVTIKIWDIGGQ PRFRSMWERYCRGVNAIVYMIDAAD REKIEASRNELHNLLDKPQLQGIPV LVLGNKRDLPNALDEKQLIEKMNLS AIQDREICCYSISCKEKDNIDITLQ WLIQHSKSRRS
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Stability: Store for up to 12 months at -20°C to -80°C as lyophilized powder.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.

Gene Information

• Gene Name: ARL8B ADP-ribosylation factor-like 8B [ Homo sapiens ]
• Official Symbol: ARL8B
• Synonyms: ARL8B; ADP-ribosylation factor-like 8B; ADP ribosylation factor like 10C , ARL10C; ADP-ribosylation factor-like protein 8B; FLJ10702; Gie1; ADP-ribosylation factor-like 10C; ADP-ribosylation factor-like protein 10C; novel small G protein indispensable for equal chromosome segregation 1; ARL10C
• Gene ID: 55207
• mRNA Refseq: NM_018184
• Protein Refseq: NP_060654
• UniProt ID: Q9NVJ2

Reviews

04/15/2023

The manufacturer also ensures that the ARL8B protein is well-characterized, with extensive documentation on its functional properties.

06/18/2022

the ease of use and compatibility of the ARL8B protein with various techniques further enhances its appeal.

01/26/2022

It has become an indispensable tool in my experiments, consistently meeting my needs and providing valuable insights.

10/16/2021

With its well-characterized nature and availability in various formats, the arnC protein is an advantageous tool that aids researchers in achieving their scientific goals.

12/31/2020

the ARL8B protein is available in different formats, including recombinant and purified variants.

07/07/2020

This flexibility allows researchers to select the most suitable form for their particular experimental setup, enhancing the efficiency and accuracy of their trials.

05/24/2019

the ARL8B protein stands out for its excellent quality, exceptional technical support, and wide range of applications.

06/26/2017

The ARL8B protein is a top-quality product that is sure to fulfill my experimental requirements.

Q&As

Can ARL8B affect cellular metabolism?

There are indications that ARL8B can influence cellular metabolism. It has been shown to regulate autophagy, a process involved in cellular recycling and metabolism. Modulation of ARL8B activity may impact metabolic processes, although further research is needed to fully understand its role in cellular metabolism.

Does ARL8B have any specific interacting partners?

ARL8B interacts with various proteins to carry out its functions. For example, it interacts with SKIP (SifA and kinesin-interacting protein) to regulate lysosomal trafficking and fusion with endocytic compartments.

Does ARL8B play a role in immune response?

Yes, ARL8B has been implicated in immune response pathways. It influences the trafficking and maturation of immune-related vesicles, such as phagolysosomes, which are involved in the processing of microbial pathogens.

Does ARL8B contribute to neuronal development or function?

While the specific role of ARL8B in neuronal development or function has not been extensively studied, its involvement in lysosomal trafficking and autophagy suggests it may have implications for neuronal homeostasis and pathology. Further research is needed to explore this connection.

Are there any known post-translational modifications of the ARL8B protein?

Yes, ARL8B can undergo various post-translational modifications, such as phosphorylation and ubiquitination, which can regulate its activity and interactions with other cellular components.

Can aberrant ARL8B expression contribute to disease development?

Dysregulation of ARL8B expression or function has been implicated in certain diseases. For example, altered ARL8B expression has been observed in cancer cells and neurodegenerative disorders, suggesting its potential involvement in disease progression.

Does ARL8B have any roles in cellular homeostasis?

Yes, ARL8B is involved in maintaining cellular homeostasis by regulating processes such as lysosomal function, vesicle trafficking, autophagy, and endocytic recycling. Disruption of ARL8B function can lead to imbalances in these processes, potentially affecting cellular homeostasis.

Are there any known mutations or polymorphisms in the ARL8B gene?

Limited information is available on mutations or polymorphisms in the ARL8B gene. However, like other genes, variations such as single nucleotide polymorphisms (SNPs) may exist in the population, and their functional significance requires further investigation.

Are there any known ARL8B animal models?

Yes, there are ARL8B knockout mouse models that have been generated to study its functions in vivo. These models provide valuable insights into the physiological roles of ARL8B and its implications in disease.

Is ARL8B expression tissue-specific?

ARL8B is expressed in various tissues and cell types. Its expression levels may vary among different tissues, indicating potential tissue-specific regulation or functions.

Can ARL8B play a role in autophagy?

Yes, ARL8B is involved in autophagy, promoting the fusion of autophagosomes with lysosomes for cargo degradation. It is essential for autophagosome-lysosome fusion and proper autophagic flux.

Does ARL8B have any roles in intracellular vesicle transport?

Yes, ARL8B plays a crucial role in intracellular vesicle transport. It facilitates the movement of vesicles along microtubules and regulates their fusion with target membranes, ensuring proper cargo delivery and organelle homeostasis.

Are there any drugs or therapeutic interventions targeting ARL8B?

Currently, there are no specific drugs or therapeutic interventions targeting ARL8B. However, as our understanding of its role in cellular processes and diseases advances, it may become a potential target for future drug development.

Can ARL8B be used as a target for drug delivery or nanomedicine?

ARL8B's involvement in lysosomal trafficking and autophagy pathways suggests it may have potential as a target for drug delivery or nanomedicine approaches. Modulating ARL8B function could potentially enhance cargo delivery to specific cellular compartments. However, further research is needed to explore this possibility.

Can ARL8B be targeted for therapeutic purposes?

Targeting ARL8B for therapeutic purposes is an area of ongoing research. Given its involvement in cellular processes relevant to diseases, modulating ARL8B activity or interactions may hold potential for future therapeutic interventions.

Does ARL8B have any implications in neurodegenerative diseases?

There is emerging evidence linking ARL8B to neurodegenerative diseases such as Parkinson's disease. Altered ARL8B expression or function may disrupt lysosomal trafficking and autophagy, leading to accumulation of protein aggregates and neurotoxicity.