Recombinant Human ARPC1A 293 Cell Lysate
Cat.No. : | ARPC1A-8687HCL |
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Description : | Antigen standard for actin related protein 2/3 complex, subunit 1A, 41kDa (ARPC1A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ARPC1A actin related protein 2/3 complex, subunit 1A, 41kDa [ Homo sapiens ] |
Official Symbol : | ARPC1A |
Synonyms : | ARPC1A; actin related protein 2/3 complex, subunit 1A, 41kDa; actin related protein 2/3 complex, subunit 1A (41 kD); actin-related protein 2/3 complex subunit 1A; actin binding protein (Schizosaccharomyces pombe sop2 like); Arc40; SOP2 like protein; SOP2Hs; SOP2L; SOP2-like protein; actin binding protein (Schizosaccharomyces pombe sop2-like); |
Gene ID : | 10552 |
mRNA Refseq : | NM_001190996 |
Protein Refseq : | NP_001177925 |
MIM : | 604220 |
UniProt ID : | Q92747 |
Chromosome Location : | 7q |
Pathway : | B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Pathogenic Escherichia coli infection, organism-specific biosystem; Pathogenic Escherichia coli infection, conserved biosystem; |
Function : | actin binding; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionYes, the ARPC1A protein can undergo post-translational modifications, including phosphorylation and acetylation, which may regulate its activity and interactions with other proteins. The specific effects and functional significance of these modifications are still being studied.
Yes, in addition to its association with the other subunits of the Arp2/3 complex, the ARPC1A protein can interact with various actin-binding proteins and regulatory factors. For example, it can associate with WASP (Wiskott-Aldrich syndrome protein) family members, which play a crucial role in actin cytoskeleton regulation.
The ARPC1A protein consists of multiple domains that are involved in protein-protein interactions. It has a predicted alpha-helical coiled-coil domain near the N-terminus, which mediates interactions with other subunits of the Arp2/3 complex. The C-terminus of ARPC1A contains an actin-binding-like domain (ABD) that helps anchor the complex to newly polymerized actin filaments.
The ARPC1A protein interacts with other subunits of the Arp2/3 complex, such as ARPC1B, ARPC2, and ARPC5, to form the functional complex. It also interacts with various actin-binding proteins, regulatory proteins, and signaling molecules that help regulate actin polymerization and participate in specific cellular processes.
Yes, the ARPC1A protein is expressed in most, if not all, cell types. Actin filament nucleation and branching are essential processes for various cellular functions, so the ARPC1A protein is required in a wide range of cell types and tissues.
Yes, mutations in the ARPC1A gene have been linked to a rare genetic disorder called ARPC1A-related intellectual disability, which is characterized by developmental delay, intellectual disability, and distinctive facial features. These mutations can disrupt the normal function of the ARPC1A protein, impairing actin filament nucleation and branching processes.
The activity and expression of the ARPC1A protein can be regulated in multiple ways. It can be regulated at the transcriptional level through various transcription factors and signaling pathways. Post-translational modifications, such as phosphorylation and acetylation, can also regulate its activity. Additionally, the availability of other subunits of the Arp2/3 complex can influence the assembly and stability of the complex, thereby affecting ARPC1A function.
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