Recombinant Human ARPP19 293 Cell Lysate

• Cat.No.: ARPP19-8681HCL

Specification

• Description: Antigen standard for cAMP-regulated phosphoprotein, 19kDa (ARPP19) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ARPP19 cAMP-regulated phosphoprotein, 19kDa [ Homo sapiens(human) ]
• Official Symbol: ARPP19
• Synonyms: ARPP19; ENSAL; ARPP16; ARPP-16; ARPP-19; cAMP-regulated phosphoprotein, 19kDa; cAMP-regulated phosphoprotein 19; cyclic AMP phosphoprotein, 19 kD
• Gene ID: 10776
• mRNA Refseq: NM_006628
• Protein Refseq: NP_006619
• MIM: 605487
• UniProt ID: P56211
• Chromosome Location: 15q21.2
• Pathway: Cell Cycle; M Phase; MASTL Facilitates Mitotic Progression; Mitotic Prophase
• Function: phosphatase inhibitor activity; potassium channel regulator activity; protein phosphatase 2A binding; protein phosphatase inhibitor activity

Reviews

01/22/2021

The knowledgeable and experienced support team is readily available to address any questions or concerns that researchers may have throughout their experimental journey.

11/10/2018

Its stability and reliability warrant its inclusion in my research protocols, leading to meaningful and conclusive results.

01/25/2017

One remarkable aspect of working with the ARPP19 protein is the excellent technical support provided by its manufacturer.

Q&As

Is the expression of ARPP19 protein regulated during development or in response to specific stimuli?

The regulation of ARPP19 expression is not extensively studied. However, some studies have suggested that its expression can be modulated in response to various signaling pathways, hormones, and stress conditions.

Are there any known interacting partners of ARPP19 protein?

Yes, ARPP19 has been found to interact with several proteins, including PP1, calcineurin, GAPDH, NudC, and NudCL2. These interactions might contribute to the modulation of PP1 activity and other cellular processes.

Can ARPP19 protein form homo- or hetero-multimers?

ARPP19 protein has been observed to form homodimers, meaning it can bind to itself and create dimers. This dimerization might play a role in its function and regulation.

Are there any known diseases or conditions associated with abnormalities in ARPP19 protein expression or function?

To date, no specific diseases or conditions have been directly linked to abnormalities in ARPP19 protein expression or function. However, further research is necessary to explore any potential associations.

Does the ARPP19 protein have any known post-translational modifications?

Yes, ARPP19 can undergo phosphorylation at specific sites, which might regulate its activity or interactions with other proteins. However, the exact functional consequences of these modifications are still under investigation.