Recombinant human ARPP19, His-tagged

• Cat.No.: ARPP19-2449H
• Product Overview: Recombinant human ARPP19 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: cAMP-regulated phosphoprotein 19, also known as ARPP19, is protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. This protein may indirectly enhance GAP-43 expression.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
• Molecular Mass: 14.7kDa (135aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by BCA assay)

Gene Information

• Gene Name: ARPP19?cAMP-regulated phosphoprotein, 19kDa [?Homo sapiens(human) ]
• Official Symbol: ARPP19
• Synonyms: ARPP19; ENSAL; ARPP16; ARPP-16; ARPP-19; cAMP-regulated phosphoprotein, 19kDa; cAMP-regulated phosphoprotein 19; cyclic AMP phosphoprotein, 19 kD
• Gene ID: 10776
• mRNA Refseq: NM_006628
• Protein Refseq: NP_006619
• MIM: 605487
• UniProt ID: P56211
• Chromosome Location: 15q21.2
• Pathway: Cell Cycle; M Phase; MASTL Facilitates Mitotic Progression; Mitotic Prophase
• Function: phosphatase inhibitor activity; potassium channel regulator activity; protein phosphatase 2A binding; protein phosphatase inhibitor activity

Reviews

01/22/2021

The knowledgeable and experienced support team is readily available to address any questions or concerns that researchers may have throughout their experimental journey.

11/10/2018

Its stability and reliability warrant its inclusion in my research protocols, leading to meaningful and conclusive results.

01/25/2017

One remarkable aspect of working with the ARPP19 protein is the excellent technical support provided by its manufacturer.

Q&As

Is the expression of ARPP19 protein regulated during development or in response to specific stimuli?

The regulation of ARPP19 expression is not extensively studied. However, some studies have suggested that its expression can be modulated in response to various signaling pathways, hormones, and stress conditions.

Are there any known interacting partners of ARPP19 protein?

Yes, ARPP19 has been found to interact with several proteins, including PP1, calcineurin, GAPDH, NudC, and NudCL2. These interactions might contribute to the modulation of PP1 activity and other cellular processes.

Can ARPP19 protein form homo- or hetero-multimers?

ARPP19 protein has been observed to form homodimers, meaning it can bind to itself and create dimers. This dimerization might play a role in its function and regulation.

Are there any known diseases or conditions associated with abnormalities in ARPP19 protein expression or function?

To date, no specific diseases or conditions have been directly linked to abnormalities in ARPP19 protein expression or function. However, further research is necessary to explore any potential associations.

Does the ARPP19 protein have any known post-translational modifications?

Yes, ARPP19 can undergo phosphorylation at specific sites, which might regulate its activity or interactions with other proteins. However, the exact functional consequences of these modifications are still under investigation.