Recombinant Human ARSJ 293 Cell Lysate
Cat.No. : | ARSJ-8674HCL |
- Specification
- Gene Information
- Related Products
- Download
Description : | Antigen standard for arylsulfatase family, member J (ARSJ) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ARSJ arylsulfatase family, member J [ Homo sapiens ] |
Official Symbol : | ARSJ |
Gene ID : | 79642 |
mRNA Refseq : | NM_024590.3 |
Protein Refseq : | NP_078866.3 |
MIM : | 610010 |
UniProt ID : | Q5FYB0 |
Chromosome Location : | 4q26 |
Pathway : | Glycosphingolipid metabolism, organism-specific biosystem;Metabolism, organism-specific biosystem;Metabolism of lipids and lipoproteins, organism-specific biosystem;Metabolism of proteins, organism-specific biosystem;PTM: gamma carboxylation, hypusine formation and arylsulfatase activation, organism-specific biosystem;Phospholipid metabolism, organism-specific biosystem;Post-translational protein modification, organism-specific biosystem; |
Function : | arylsulfatase activity;metal ion binding; |
Products Types
◆ Recombinant Protein | ||
ARSJ-867H | Recombinant Human ARSJ protein, GST-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionCurrently, there is limited information on animal models specifically targeting the ARSJ protein. However, studies using various animal models have provided insights into the molecular processes and associated diseases affected by altered ARSJ function.
The ARSJ protein is typically found in the lysosomes, which are membrane-bound compartments within cells responsible for breaking down various molecules.
Yes, the ARSJ protein is conserved among different species, indicating its importance in sulfate metabolism throughout evolution.
Currently, there are no specific therapies targeting the ARSJ protein. However, further research may identify potential therapeutic approaches that could modulate ARSJ function in diseases associated with sulfate metabolism disturbances.
Yes, the ARSJ protein has been shown to interact with other proteins involved in sulfate metabolism, such as ARSI (arylsulfatase I) and ARSK (arylsulfatase K). These interactions likely play a role in coordinating sulfate removal in various tissues.
The regulatory mechanisms controlling ARSJ protein expression and activity are not fully understood. However, research suggests that transcriptional regulation and post-translational modifications may influence its expression and enzymatic activity.
Mutations in the ARSJ gene have been linked to a rare genetic disorder called X-linked chondrodysplasia punctata 1 (CDPX1), which is characterized by skeletal abnormalities and developmental defects.
Customer Reviews (3)
Write a reviewI highly recommend the ARSJ protein for various experimental applications.
It has demonstrated outstanding performance in ELISA, providing reliable and precise results.
I am certain that the ARSJ protein will play a pivotal role in facilitating robust and insightful research, providing the assurance of reliable and consistent results.
Ask a Question for All ARSJ Products
Required fields are marked with *
My Review for All ARSJ Products
Required fields are marked with *
Inquiry Basket