Recombinant Human ARSK protein, GST-tagged

• Cat.No.: ARSK-868H
• Product Overview: Human ARSK full-length ORF ( NP_937793.1, 1 a.a. - 536 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Sulfatases (EC 3.1.5.6), such as ARSK, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules (Sardiello et al., 2005 [PubMed 16174644]).[supplied by OMIM, Mar 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 87.9 kDa
• AA Sequence: MLLLWVSVVAALALAVLAPGAGEQR RRAAKAPNVVLVVSDSFDGRLTFHP GSQVVKLPFINFMKTRGTSFLNAYT NSPICCPSRAAMWSGLFTHLTESWN NFKGLDPNYTTWMDVMERHGYRTQK FGKLDYTSGHHSISNRVEAWTRDVA FLLRQEGRPMVNLIRNRTKVRVMER DWQNTDKAVNWLRKEAINYTEPFVI YLGLNLPHPYPSPSSGENFGSSTFH TSLYWLEKVSHDAIKIPKWSPLSEM HPVDYYSSYTKNCTGRFTKKEIKNI RAFYYAMCAETDAMLGEIILALHQL DLLQKTIVIYSSDHGELAMEHRQFY KMSMYEASAHVPLLMMGPGIKAGLQ VSNVVSLVDIYPTMLDIAGIPLPQN LSGYSLLPLSSETFKNEHKVKNLHP PWILSEFHGCNVNASTYMLRTNHWK YIAYSDGASILPQLFDLSSDPDELT NVAVKFPEITYSLDQKLHSIINYPK VSASVHQYNKEQFIKWKQSIGQNYS NVIANLRWHQDWQKEPRKYENAIDQ WLKTHMNPRAV
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARSK arylsulfatase family, member K [ Homo sapiens ]
• Official Symbol: ARSK
• Synonyms: ARSK; arylsulfatase family, member K; arylsulfatase K; DKFZp313G1735; TSULF; ASK; telethon sulfatase
• Gene ID: 153642
• mRNA Refseq: NM_198150
• Protein Refseq: NP_937793
• MIM: 610011
• UniProt ID: Q6UWY0

Reviews

10/14/2022

When used in Western Blotting experiments, the ARSK protein consistently generates clear and distinct protein bands.

07/16/2018

It has been invaluable in protein electron microscopy structure analysis, facilitating detailed investigations and yielding valuable insights.

01/24/2018

This exceptional clarity allows for accurate quantification and analysis, contributing to the overall success of my research endeavors.

Q&As

Are there any known natural substrates for the ARSK protein?

The ARSK protein is known to hydrolyze a specific class of sulfates known as sulfatides. Sulfatides are a type of glycosphingolipid found in various tissues and cells, particularly in the nervous system. ARSK catalyzes the breakdown of sulfatides into sulfate ions and ceramide.

Is the ARSK gene subject to genetic variations or mutations?

Yes, genetic variations or mutations in the ARSK gene have been reported in individuals with ARSK-related disorders. These variations can interfere with the proper functioning of the enzyme, leading to impaired sulfate metabolism and the associated clinical symptoms.

What happens if there is a deficiency or dysfunction of the ARSK protein?

Deficiency or dysfunction of the ARSK protein can lead to a rare genetic disorder called ARSK-related disorder. This condition is characterized by the accumulation of sulfatides, a type of lipid, in various organs and tissues, leading to tissue damage and dysfunction.

What are the implications of ARSK dysfunction on neurodegenerative diseases?

Some studies have suggested that ARSK dysfunction and subsequent sulfatide accumulation may contribute to the pathogenesis of certain neurodegenerative diseases, such as multiple sclerosis and metachromatic leukodystrophy. The deposition of sulfatides in neural tissues may disrupt myelin integrity and impair nerve conduction.

Are there any known treatments or therapies for ARSK-related disorders?

Currently, there are no specific treatments or therapies available for ARSK-related disorders. Management typically involves symptomatic and supportive care to address the specific symptoms and complications that arise due to sulfatide accumulation.

Can genetic testing be used to diagnose ARSK-related disorders?

Yes, genetic testing can be used to diagnose ARSK-related disorders. By sequencing the ARSK gene and identifying disease-causing variations or mutations, a definitive diagnosis can be made. Genetic counseling may also be offered to affected individuals and their families.

What are the potential long-term effects of ARSK dysfunction?

The long-term effects of ARSK dysfunction may vary depending on the severity and specific manifestations of the disorder. Sulfatide accumulation can lead to damage in various organs, including the brain, liver, and kidneys, resulting in neurological, hepatic, or renal impairments over time.

Is the ARSK protein implicated in any other diseases or conditions?

Currently, there is limited research on whether the ARSK protein is implicated in other diseases or conditions. Further studies are needed to determine if it plays a role in other aspects of sulfate metabolism or if dysregulation of ARSK is associated with other disorders.

Are there any known inhibitors or activators of the ARSK protein?

At present, no specific inhibitors or activators of the ARSK protein have been identified. The development of small molecules or compounds that can modulate ARSK activity could hold potential for future therapeutic interventions, but more research is needed in this area.

How is the ARSK protein regulated in the body?

The regulation of the ARSK protein is not fully understood. It is likely controlled at the transcriptional level, where the expression of the ARSK gene is influenced by various regulatory factors. Additionally, post-translational modifications and protein-protein interactions may also participate in the regulation of ARSK activity.