Recombinant Human ASAP2 293 Cell Lysate

• Cat.No.: ASAP2-8667HCL

Specification

• Description: Antigen standard for ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASAP2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Homo sapiens ]
• Official Symbol: ASAP2
• Synonyms: ASAP2; ArfGAP with SH3 domain, ankyrin repeat and PH domain 2; DDEF2, development and differentiation enhancing factor 2; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2; centaurin; beta 3; CENTB3; KIAA0400; PAP; SHAG1; centaurin, beta 3; PYK2 C terminus-associated protein; pyk2 C-terminus-associated protein; development and differentiation enhancing factor 2; development and differentiation-enhancing factor 2; paxillin-associated protein with ARF GAP activity 3; PAG3; AMAP2; DDEF2; Pap-alpha; FLJ42910
• Gene ID: 8853
• mRNA Refseq: NM_001135191
• Protein Refseq: NP_001128663
• MIM: 603817
• UniProt ID: O43150
• Chromosome Location: 2p24
• Pathway: Arf6 trafficking events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem
• Function: ARF GTPase activator activity; enzyme activator activity; metal ion binding; protein binding; zinc ion binding

Reviews

05/23/2022

The ASAP2 protein exhibits outstanding performance in enzyme-linked immunosorbent assays (ELISA).

11/13/2021

The protein's reliability and reproducibility in WB ensure accurate analysis of target proteins, while its suitability for electron microscopy structure analysis enables detailed examination of molecular structures.

11/16/2020

The versatility and efficacy of ASAP2 in these applications make it an invaluable tool for researchers.

Q&As

Where is ASAP2 primarily localized within cells?

ASAP2 is found in the cytoplasm, associated with cellular membranes, particularly the Golgi apparatus and endosomes.

Can ASAP2 be regulated by post-translational modifications?

Yes, ASAP2 can undergo phosphorylation and ubiquitination, which may influence its stability, activity, and protein-protein interactions.

Can ASAP2 interact with other proteins?

Yes, ASAP2 can interact with various proteins involved in membrane trafficking, including clathrin, AP1, AP3, and many others.

Is ASAP2 involved in cancer progression?

ASAP2 has been implicated in cancer metastasis due to its ability to promote cell migration and invasion through its effect on the actin cytoskeleton.

How does ASAP2 regulate membrane trafficking?

ASAP2 promotes the hydrolysis of GTP bound to Arf1, leading to the inactivation of Arf1 and subsequent modulation of vesicle budding, cargo sorting, and membrane remodeling.

What are the domains present in ASAP2?

ASAP2 contains an SH3 domain, an ANK repeat domain, and a PH (pleckstrin homology) domain.

Are there any genetic variations associated with ASAP2?

Genetic variations in ASAP2 have been identified in some studies, and they may be linked to certain diseases or phenotypes, although further research is needed to establish concrete associations.

Does ASAP2 play a role in cell adhesion?

Yes, ASAP2 has been suggested to modulate cell adhesion and migration by regulating actin cytoskeleton dynamics through its interaction with Rho family GTPases.