Recombinant Human ASB4 293 Cell Lysate

• Cat.No.: ASB4-8663HCL

Specification

• Description: Antigen standard for ankyrin repeat and SOCS box-containing 4 (ASB4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASB4 ankyrin repeat and SOCS box containing 4 [ Homo sapiens ]
• Official Symbol: ASB4
• Synonyms: ASB4; ankyrin repeat and SOCS box containing 4; ankyrin repeat and SOCS box protein 4; ASB 4; ankyrin repeat and SOCS box-containing 4; ASB-4; MGC142039; MGC142041
• Gene ID: 51666
• mRNA Refseq: NM_145872
• Protein Refseq: NP_665879
• MIM: 605761
• UniProt ID: Q9Y574
• Chromosome Location: 7q21-q22
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem

Reviews

01/30/2023

The manufacturer's outstanding technical support serves as a valuable resource, providing prompt and knowledgeable assistance whenever I encounter difficulties or require guidance.

07/27/2020

the ASB4 protein's exceptional quality and purity guarantee its suitability for a wide range of experimental applications.

04/03/2016

Its consistent performance and remarkable stability make it a reliable tool in studying various cellular processes and signaling pathways.

Q&As

Can ASB4 regulate the degradation of specific target proteins?

Yes, ASB4 interacts with specific target proteins, recognizing them and facilitating their ubiquitination, leading to their subsequent degradation.

Can ASB4 have both cytoplasmic and nuclear functions?

Yes, ASB4 has been observed in both the cytoplasm and the nucleus, suggesting its involvement in diverse cellular compartments and functions.

Is the ASB4 protein conserved across different species?

Yes, ASB4 shows evolutionary conservation, indicating its functional importance across various species.

Does ASB4 have any known substrate specificity?

Yes, ASB4 selectively targets certain proteins for degradation, usually by recognizing specific recognition motifs or interacting with other regulatory factors.

Are there any known interacting proteins that modulate ASB4 activity?

ASB4 can interact with various proteins, including its ubiquitin ligase complex partners or substrate proteins, influencing its activity, localization, or substrate specificity.

Are there any diseases or disorders associated with ASB4 dysfunction?

Currently, there are no known diseases or disorders directly linked to ASB4 dysfunction. However, its dysregulation may potentially contribute to various pathological conditions, requiring further investigation.

What are some of the cellular processes influenced by ASB4-mediated protein degradation?

ASB4-mediated protein degradation can impact cellular processes such as gene expression, signal transduction, cell proliferation, and differentiation.

Can ASB4 play a role in muscle development or function?

Yes, ASB4 has been implicated in skeletal muscle development, differentiation, and regeneration processes based on experimental evidence and studies in animal models.

Are there any drugs or therapeutic interventions targeting ASB4?

Presently, there are no specific drugs targeting ASB4. However, understanding its role and identifying potential modulators may open avenues for therapeutic interventions in the future.

Does ASB4 expression respond to external signals or stimuli?

Yes, ASB4 expression can be regulated by external signals, including hormones, cytokines, growth factors, and cellular stress.