Recombinant Human ASB6 293 Cell Lysate

• Cat.No.: ASB6-8661HCL

Specification

• Description: Antigen standard for ankyrin repeat and SOCS box-containing 6 (ASB6), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ASB6 ankyrin repeat and SOCS box containing 6 [ Homo sapiens ]
• Official Symbol: ASB6
• Synonyms: ASB6; ankyrin repeat and SOCS box containing 6; ankyrin repeat and SOCS box protein 6; ankyrin repeat and SOCS box-containing 6; MGC1024; FLJ20548
• Gene ID: 140459
• mRNA Refseq: NM_001202403
• Protein Refseq: NP_001189332
• UniProt ID: Q9NWX5
• Chromosome Location: 9q34.13
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem

Reviews

04/08/2021

Its high specificity and robust activity make it an ideal candidate for investigating intricate molecular interactions and elucidating complex biological mechanisms.

07/08/2019

This variety allows me to select the most appropriate option based on the specific requirements of my research.

07/17/2018

With its remarkable quality, reliability, and the manufacturer's excellent technical support, I am confident that it will be an invaluable asset in driving my scientific inquiries forward and facilitating groundbreaking discoveries in my field.

Q&As

Are there any known molecular interactions involving ASB6?

The molecular interactions involving ASB6 are not well-characterized. Identifying its binding partners or protein substrates would provide insights into its functional mechanisms.

Are there any known upstream regulators that can modulate ASB6 expression?

The specific upstream regulators that modulate ASB6 expression have not been extensively investigated. Further studies are needed to identify the regulatory mechanisms involved.

Can ASB6 be used as a diagnostic marker for any diseases or conditions?

Currently, ASB6 has not been identified as a diagnostic marker for any specific diseases or conditions. Further investigations may reveal its potential diagnostic applicability.

Is there any evidence to suggest a role for ASB6 in cancer development?

There is limited research on the role of ASB6 in cancer development. While some studies have suggested a possible involvement of ASB6 in certain types of cancer, additional research is required to establish a definitive link.