Recombinant Human ATP2B1 protein, GST-tagged

• Cat.No.: ATP2B1-972H
• Product Overview: Human ATP2B1 partial ORF ( NP_001673, 1 a.a. - 97 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 36.41 kDa
• AA Sequence: MGDMANNSVAYSGVKNSLKEANHDG DFGITLAELRALMELRSTDALRKIQ ESYGDVYGICTKLKTSPNEGLSGNP ADLERREAVFGKNFIPPKKPKT
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ATP2B1 ATPase, Ca++ transporting, plasma membrane 1 [ Homo sapiens ]
• Official Symbol: ATP2B1
• Synonyms: ATP2B1; ATPase, Ca++ transporting, plasma membrane 1; plasma membrane calcium-transporting ATPase 1; plasma membrane calcium transporting ATPase 1; PMCA1; plasma membrane calcium pump; PMCA1kb
• Gene ID: 490
• mRNA Refseq: NM_001001323
• Protein Refseq: NP_001001323
• MIM: 108731
• UniProt ID: P20020

Reviews

03/14/2022

The bands of ATP2B1 in Western blot were regular in shape and showed good quality.

08/11/2021

ATP2B1 is really amazing. It maintains a very stable performance every time it is used. You can see that its production process is very careful.

10/06/2020

The high catalytic efficiency, safety, and expression level of this protein make it ideal for laboratory and practical applications.

Q&As

What are the pathways and forms of human ATP2Brecombinant proteins?

Human ATP2B1 recombinant protein can be administered by injection, intravenous drip, and other ways, which can be solution, powder or lyophilized powder.

What research has been carried out on human ATP2Brecombinant protein?

Previous studies have focused on the mechanism of ATP2B1 protein in hypertension and cardiovascular disease, the correlation of polymorphism, and the relationship between ATP2B1 variation and disease susceptibility.

What is the main mechanism of action of the human recombinant ATP2Bprotein?

ATP2B1 recombinant protein may affect physiological functions such as vasoconstriction and cardiac contraction by regulating calcium transport, thereby affecting hypertension and cardiovascular diseases.

What is the relationship between human ATP2Bgene expression and disease?

Some studies have found that the expression level of ATP2B1 gene is related to hypertension, heart disease and other diseases, but the detailed mechanism needs to be further explored and confirmed.

How is the therapeutic potential of the human recombinant ATP2Bprotein evaluated?

The evaluation of the therapeutic potential of human ATP2B1 recombinant protein mainly includes in vitro experiments, animal model studies and clinical trials.

Are there other related proteins that function similarly to ATP2B1?

Yes, in addition to ATP2B1, other calcium transporters (such as ATP2A1 and ATP2A2) have been found to be related to the regulation of intracellular and extracellular calcium balance.