Recombinant Human ATP2B3 Protein, His-tagged

• Cat.No.: ATP2B3-267H
• Product Overview: Recombinant Human ATP2B3 Protein (Thr1057-Leu1220) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.1 kDa
  Accurate Molecular Mass: 25 kDa
• Protein length: Thr1057-Leu1220
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: ATP2B3 ATPase, Ca++ transporting, plasma membrane 3 [ Homo sapiens (human) ]
• Official Symbol: ATP2B3
• Synonyms: ATP2B3; ATPase, Ca++ transporting, plasma membrane 3; plasma membrane calcium-transporting ATPase 3; plasma membrane calcium transporting ATPase 3; PMCA3; plasma membrane calcium pump; plasma membrane calcium ATPase; PMCA3a
• Gene ID: 492
• mRNA Refseq: NM_001001344
• Protein Refseq: NP_001001344
• MIM: 300014
• UniProt ID: Q16720

Reviews

06/28/2022

ATP2B3 were of very high quality and reliable, which is very helpful to our results.

04/08/2022

Due to the high purity of ATP2B3, its production process is very quality-oriented.

12/03/2019

The reproducibility of ATP2B3 is very good, and the results of each experiment are very consistent, indicating that its production process is very careful.

Q&As

How is the protein structure and function of ATP2B3 different from other atpase B family members?

There are several differences in protein structure and function between ATP2B3 and other atpase B family members, mainly depending on how they are expressed and regulated in different tissues and cells.

Are mutations in ATP2B3 associated with rare genetic diseases?

ATP2B3 gene mutation may be associated with some hereditary diseases such as pigmentary papillomatosis and atrial fibrillation/flutte

Is ATP2B3 associated with neurological diseases?

At present, studies have shown that ATP2B3 is associated with nervous system diseases such as Parkinson's disease and Alzheimer's disease.

What are the pathways of ATP2B3 protein production?

The production pathway of ATP2B3 protein mainly includes multiple biological processes such as gene transcription, RNA splicing, protein translation and subsequent modification.

What is the regulatory mechanism of ATP2B3?

The regulatory mechanism of ATP2B3 may involve multiple signaling pathways and factors, such as calcium ions, ATP, membrane receptors, etc.

What is the relationship between ATP2B3 and cardiovascular disease?

The relationship between ATP2B3 and cardiovascular disease may be achieved through several mechanisms such as regulating cardiomyocyte contraction and vascular smooth muscle cell contraction.