Recombinant Human ATP5A1 protein, GST-tagged

• Cat.No.: ATP5A1-976H
• Product Overview: Human ATP5A1 full-length ORF ( NP_001001937.1, 1 a.a. - 553 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 86.2 kDa
• AA Sequence: MLSVRVAAAVVRALPRRAGLVSRNA LGSSFIAARNFHASNTHLQKTGTAE MSSILEERILGADTSVDLEETGRVL SIGDGIARVHGLRNVQAEEMVEFSS GLKGMSLNLEPDNVGVVVFGNDKLI KEGDIVKRTGAIVDVPVGEELLGRV VDALGNAIDGKGPIGSKTRRRVGLK APGIIPRISVREPMQTGIKAVDSLV PIGRGQRELIIGDRQTGKTSIAIDT IINQKRFNDGSDEKKKLYCIYVAIG QKRSTVAQLVKRLTDADAMKYTIVV SATASDAAPLQYLAPYSGCSMGEYF RDNGKHALIIYDDLSKQAVAYRQMS LLLRRPPGREAYPGDVFYLHSRLLE RAAKMNDAFGGGSLTALPVIETQAG DVSAYIPTNVISITDGQIFLETELF YKGIRPAINVGLSVSRVGSAAQTRA MKQVAGTMKLELAQYREVAAFAQFG SDLDAATQQLLSRGVRLTELLKQGQ YSPMAIEEQVAVIYAGVRGYLDKLE PSKITKFENAFLSHVVSQHQALLGT IRADGKISEQSDAKLKEIVTNFLAG FEA
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ATP5A1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle [ Homo sapiens ]
• Official Symbol: ATP5A1
• Synonyms: ATP5A1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle , ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non cardiac muscle like 2 , ATP5AL2, ATPM; ATP synthase subunit alpha, mitochondrial; ATP5A; hATP1; OMR; ORM; ATP sythase (F1-ATPase) alpha subunit; ATP synthase alpha chain, mitochondrial; mitochondrial ATP synthetase, oligomycin-resistant; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATPM; MOM2; ATP5AL2
• Gene ID: 498
• mRNA Refseq: NM_001001935
• Protein Refseq: NP_001001935
• MIM: 164360
• UniProt ID: P25705

Reviews

08/04/2020

It is not easy to be contaminated, and then stored.

11/29/2019

The reproducibility of ATP5A1 is very good, with very consistent results in every experiment or application, benefiting from its high degree of stability and reproducibility.

05/30/2019

Using ATP5A1 as a catalyst, the rate and yield of chemical reactions can be significantly increased with high catalytic efficiency.

Q&As

What other proteins does ATP5Ainteract with?

ATP5A1 interacts with other ATP synthase subunits and a variety of mitochondrial proteins (such as NADH dehydrogenase and chromosomal acyl-coa carboxylase) to participate in the process of energy metabolism.

Is the abnormal expression of ATP5Avaluable in the diagnosis and prognosis of neurodegenerative diseases?

The abnormal expression of ATP5A1 can be used as an important indicator for the diagnosis and prognosis of some neurodegenerative diseases, but it needs to be comprehensively analyzed in combination with other clinical and molecular biological results.

What is the mutation mechanism of ATP5A1?

Mutations in ATP5A1 may lead to abnormal changes in its intracellular localization and function, thereby affecting mitochondrial neuroregulation and energy metabolism.

What is the role of ATP5Ain biological development?

ATP5A1 participates in the regulation of cellular energy metabolism and mitochondrial function, and plays an important role in cell growth and development.

Are there any studies on drugs for ATP5Aregulating cellular energy metabolism?

Yes, several studies have been devoted to the development of drugs that can regulate the activity of ATP5A1 to affect cellular energy metabolism and treat related diseases.

Is the expression pattern of ATP5Aassociated with liver cancer or other tumors?

Yes, some studies have found that the expression pattern of ATP5A1 is related to the malignant degree and prognosis of liver cancer, bladder cancer, prostate cancer and other tumors.