Recombinant Human ATP5A1 protein, His-tagged

• Cat.No.: ATP5A1-3983H
• Product Overview: Recombinant Human ATP5A1 protein(202-553 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 202-553 aa
• AA Sequence: IGRGQRELIIGDRQTGKTSIAIDTI INQKRFNDGSDEKKKLYCIYVAIGQ KRSTVAQLVKRLTDADAMKYTIVVS ATASDAAPLQYLAPYSGCSMGEYFR DNGKHALIIYDDLSKQAVAYRQMSL LLRRPPGREAYPGDVFYLHSRLLER AAKMNDAFGGGSLTALPVIETQAGD VSAYIPTNVISITDGQIFLETELFY KGIRPAINVGLSVSRVGSAAQTRAM KQVAGTMKLELAQYREVAAFAQFGS DLDAATQQLLSRGVRLTELLKQGQY SPMAIEEQVAVIYAGVRGYLDKLEP SKITKFENAFLSHVVSQHQALLGTI RADGKISEQSDAKLKEIVTNFLAGF EA
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ATP5A1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle [ Homo sapiens ]
• Official Symbol: ATP5A1
• Synonyms: ATP5A1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle , ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non cardiac muscle like 2 , ATP5AL2, ATPM; ATP synthase subunit alpha, mitochondrial; ATP5A; hATP1; OMR; ORM; ATP sythase (F1-ATPase) alpha subunit; ATP synthase alpha chain, mitochondrial; mitochondrial ATP synthetase, oligomycin-resistant; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATPM; MOM2; ATP5AL2
• Gene ID: 498
• mRNA Refseq: NM_001001935
• Protein Refseq: NP_001001935
• MIM: 164360
• UniProt ID: P25705

Reviews

08/04/2020

It is not easy to be contaminated, and then stored.

11/29/2019

The reproducibility of ATP5A1 is very good, with very consistent results in every experiment or application, benefiting from its high degree of stability and reproducibility.

05/30/2019

Using ATP5A1 as a catalyst, the rate and yield of chemical reactions can be significantly increased with high catalytic efficiency.

Q&As

What other proteins does ATP5Ainteract with?

ATP5A1 interacts with other ATP synthase subunits and a variety of mitochondrial proteins (such as NADH dehydrogenase and chromosomal acyl-coa carboxylase) to participate in the process of energy metabolism.

Is the abnormal expression of ATP5Avaluable in the diagnosis and prognosis of neurodegenerative diseases?

The abnormal expression of ATP5A1 can be used as an important indicator for the diagnosis and prognosis of some neurodegenerative diseases, but it needs to be comprehensively analyzed in combination with other clinical and molecular biological results.

What is the mutation mechanism of ATP5A1?

Mutations in ATP5A1 may lead to abnormal changes in its intracellular localization and function, thereby affecting mitochondrial neuroregulation and energy metabolism.

What is the role of ATP5Ain biological development?

ATP5A1 participates in the regulation of cellular energy metabolism and mitochondrial function, and plays an important role in cell growth and development.

Are there any studies on drugs for ATP5Aregulating cellular energy metabolism?

Yes, several studies have been devoted to the development of drugs that can regulate the activity of ATP5A1 to affect cellular energy metabolism and treat related diseases.

Is the expression pattern of ATP5Aassociated with liver cancer or other tumors?

Yes, some studies have found that the expression pattern of ATP5A1 is related to the malignant degree and prognosis of liver cancer, bladder cancer, prostate cancer and other tumors.