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Recombinant Human ATP5C1 293 Cell Lysate

Cat.No. : ATP5C1-8603HCL
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Description : Antigen standard for ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 (ATP5C1), nuclear gene encoding mitochondrial protein, transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : ATP5C1 ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 [ Homo sapiens ]
Official Symbol : ATP5C1
Synonyms : ATP5C1; ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1; ATP5C, ATP5CL1; ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit; ATP synthase gamma chain, mitochondrial; mitochondrial ATP synthase, gamma subunit 1; ATP5C; ATP5CL1;
Gene ID : 509
mRNA Refseq : NM_005174
Protein Refseq : NP_005165
MIM : 108729
UniProt ID : P36542
Chromosome Location : 10p14
Pathway : Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; F-type ATPase, eukaryotes, organism-specific biosystem; Formation of ATP by chemiosmotic coupling, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem;
Function : contributes_to ATPase activity; contributes_to ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; proton-transporting ATPase activity, rotational mechanism; transmembrane transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the regulatory mechanisms of ATP5C1? 11/18/2021

ATP5C1 expression and function are affected by a variety of regulatory mechanisms, including regulation of transcription factors and epigenetic modifications.

Is ATP5Cexpression associated with the development of specific diseases? 10/29/2021

Yes, ATP5C1 expression levels may be associated with the development and progression of certain diseases.

In which tissues or organs is ATP5Chighly expressed? 05/23/2021

ATP5C1 is highly expressed in multiple tissues and organs, especially metabolically active tissues such as heart, liver, and brain.

Are ATP5Cgene mutations associated with nervous system diseases? 12/26/2020

Yes, ATP5C1 gene mutations are associated with certain neurological diseases such as Parkinson's disease, cerebral ischemia, etc.

Which the role of ATP5Cin tumors? 11/11/2020

It may play a role in tumor cell metabolism and energy supply.

What is the role of ATP5Cprotein in metabolic diseases? 12/19/2019

ATP5C1 protein may be related to the occurrence and progression of metabolic diseases such as obesity and diabetes.

Customer Reviews (3)

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Reviews
04/24/2022

    The exceptional stability of this protein makes it an invaluable tool for a wide range of applications.

    08/19/2021

      Due to the wide application of proteins, researchers can conduct research in more fields and broaden the horizon of scientific research.

      05/24/2019

        Because the extraordinary activity of proteins makes them valuable tools for a wide range of applications.

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