Recombinant Human ATXN1 293 Cell Lysate
Cat.No. : | ATXN1-8566HCL |
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Description : | Antigen standard for ataxin 1 (ATXN1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ATXN1 ataxin 1 [ Homo sapiens ] |
Official Symbol : | ATXN1 |
Synonyms : | ATXN1; ataxin 1; SCA1, spinocerebellar ataxia 1 (olivopontocerebellar ataxia 1, autosomal dominant, ataxin 1); ataxin-1; ATX1; D6S504E; spinocerebellar ataxia type 1 protein; SCA1; |
Gene ID : | 6310 |
mRNA Refseq : | NM_000332 |
Protein Refseq : | NP_000323 |
MIM : | 601556 |
UniProt ID : | P54253 |
Chromosome Location : | 6p23 |
Function : | DNA binding; RNA binding; identical protein binding; poly(G) RNA binding; poly(U) RNA binding; protein C-terminus binding; protein binding; protein self-association; |
Products Types
◆ Recombinant Protein | ||
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ATXN1-556R | Recombinant Rat ATXN1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Atxn1-1782M | Recombinant Mouse Atxn1 Protein, Myc/DDK-tagged | +Inquiry |
ATXN1-163H | Recombinant Human ATXN1 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
ATXN1-409H | Recombinant Human ATXN1 Protein, His (Fc)-Avi-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionSCA1 is caused by mutations in the ATXN1 gene. The mutation resulted in the expansion of glutamine repeats in ATXN1 protein, resulting in the formation of abnormal polyglutamic acid protein. This protein abnormality can lead to abnormal neuronal function and degenerative changes, leading to the occurrence of SCA1.
Yes, the ATXN1 protein interacts with multiple proteins to form complexes involved in the regulation of a variety of cellular functions. For example, ATXN1 interacts with the Capicua (CIC) protein and is involved in transcriptional regulation.
The protein is associated with spinocerebellar ataxia type 1 (SCA1). SCA1 is an inherited neurodegenerative disease characterized by dysfunction of the cerebellum and spinal cord, leading to ataxia and other neurological symptoms.
ATXN1 protein is expressed in several tissues, but its expression is higher in the central nervous system, especially in the cerebellum and spinal cord.
The function of ATXN1 protein is regulated by a variety of regulatory mechanisms, including post-translational modification, assembly of protein complexes, and transcriptional regulation. These regulatory mechanisms ensure the adaptability and stability of ATXN1 protein.
ATXN1 protein is involved in the regulation of a variety of neural cell functions, including cell cycle regulation, DNA repair, transcription regulation, etc. It plays an important role in the development and functional maintenance of the nervous system.
Customer Reviews (3)
Write a reviewUsing Atxn1 as a catalyst, the rate and yield of chemical reactions can be significantly increased, and the catalytic efficiency is very high.
The stability of Atxn1 is very good, and its structure and function remain stable even after several freeze-thaw cycles, which shows its excellent quality control and production process.
Atxn1 are so simple to prepare that we can easily prepare them ourselves.
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