Recombinant Human ATXN1 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: ATXN1-163H
• Product Overview: ATXN1 MS Standard C13 and N15-labeled recombinant protein (NP_001121636) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 40-83 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). Alternative splicing results in multiple transcript variants, with one variant encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to the use of overlapping alternate reading frames. [provided by RefSeq, Nov 2017]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 86.9 kDa
• AA Sequence: MKSNQERSNECLPPKKREIPATSRS SEEKAPTLPSDNHRVEGTAWLPGNP GGRGHGGGRHGPAGTSVELGLQQGI GLHKALSTGLDYSPPSAPRSVPVAT TLPAAYATPQPGTPVSPVQYAHLPH TFQFIGSSQYSGTYASFIPSQLIPP TANPVTSAVASAAGATTPSQRSQLE AYSTLLANMGSLSQTPGHKAEQQQQ QQQQQQQQHQHQQQQQQQQQQQQQQ HLSRAPGLITPGSPPPAQQNQYVHI SSSPQNTGRTASPPAIPVHLHPHQT MIPHTLTLGPPSQVVMQYADSGSHF VPREATKKAESSRLQQAIQAKEVLN GEMEKSRRYGAPSSADLGLGKAGGK SVPHPYESRHVVVHPSPSDYSSRDP SGVRASVMVLPNSNTPAADLEVQQA THREASPSTLNDKSGLHLGKPGHRS YALSPHTVIQTTHSASEPLPVGLPA TAFYAGTQPPVIGYLSGQQQAITYA GSLPQHLVIPGTQPLLIPVGSTDME ASGAAPAIVTSSPQFAAVPHTFVTT ALPKSENFNPEALVTQAAYPAMVQA QIHLPVVQSVASPAAAPPTLPPYFM KGSIIQLANGELKKVEDLKTEDFIQ SAEISNDLKIDSSTVERIEDSHSPG VAVIQFAVGEHRAQVSVEVLVEYPF FVFGQGWSSCCPERTSQLFDLPCSK LSVGDVCISLTLKNLKNGSVKKGQP VDPASVLLKHSKADGLAGSRHRYAE QENGINQGSAQMLSENGELKFPEKM GLPAAPFLTKIEPSKPAATRKRRWS APESRKLEKSEDEPPLTLPKPSLIP QEVKICIEGRSNVGKTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ATXN1 ataxin 1 [ Homo sapiens (human) ]
• Official Symbol: ATXN1
• Synonyms: ATXN1; ataxin 1; ATX1; D6S504E; SCA1; ataxin-1; alternative ataxin1; spinocerebellar ataxia type 1 protein
• Gene ID: 6310
• mRNA Refseq: NM_001128164
• Protein Refseq: NP_001121636
• MIM: 601556
• UniProt ID: P54253

Reviews

12/10/2020

Using Atxn1 as a catalyst, the rate and yield of chemical reactions can be significantly increased, and the catalytic efficiency is very high.

10/22/2019

The stability of Atxn1 is very good, and its structure and function remain stable even after several freeze-thaw cycles, which shows its excellent quality control and production process.

06/18/2019

Atxn1 are so simple to prepare that we can easily prepare them ourselves.

Q&As

How do mutations in ATXNprotein cause SCA1?

SCA1 is caused by mutations in the ATXN1 gene. The mutation resulted in the expansion of glutamine repeats in ATXN1 protein, resulting in the formation of abnormal polyglutamic acid protein. This protein abnormality can lead to abnormal neuronal function and degenerative changes, leading to the occurrence of SCA1.

Does ATXNprotein interact with other proteins?

Yes, the ATXN1 protein interacts with multiple proteins to form complexes involved in the regulation of a variety of cellular functions. For example, ATXN1 interacts with the Capicua (CIC) protein and is involved in transcriptional regulation.

Which diseases are associated with ATXNprotein?

The protein is associated with spinocerebellar ataxia type 1 (SCA1). SCA1 is an inherited neurodegenerative disease characterized by dysfunction of the cerebellum and spinal cord, leading to ataxia and other neurological symptoms.

In which tissues is ATXNprotein expressed?

ATXN1 protein is expressed in several tissues, but its expression is higher in the central nervous system, especially in the cerebellum and spinal cord.

What is the regulatory mechanism of ATXNprotein function?

The function of ATXN1 protein is regulated by a variety of regulatory mechanisms, including post-translational modification, assembly of protein complexes, and transcriptional regulation. These regulatory mechanisms ensure the adaptability and stability of ATXN1 protein.

What is the role of ATXNprotein in humans?

ATXN1 protein is involved in the regulation of a variety of neural cell functions, including cell cycle regulation, DNA repair, transcription regulation, etc. It plays an important role in the development and functional maintenance of the nervous system.