Recombinant human BFIT, His-tagged

• Cat.No.: BFIT-2436H
• Product Overview: Recombinant human BFIT protein, fused to His-tag at N-terminus, was expressed in E.coli

Specification

• Description: BFIT is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. The protein has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 29.9kDa (268aa)
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSNRTS RKSALRAGND SAMADGEGYR NPTEVQMSQL VLPCHTNQRG ELSVGQLLKWIDTTACLSAE RHAGCPCVTA SMDDIYFEHT ISVGQVVNIK AKVNRAFNSS MEVGIQVASE DLCSEKQWNV CKALATFVAR REITKVKLKQ ITPRTEEEKM EHSVAAERRR MRLVYADTIK DLLANCAIQG DLESRDCSRM VPAEKTRVES VELVLPPHAN HQGNTFGGQI MAWMENVA
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ACOT11?acyl-CoA thioesterase 11 [?Homo sapiens?(human) ]
• Official Symbol: ACOT11
• Synonyms: ACOT11; BFIT; THEA; THEM1; STARD14; acyl-CoA thioesterase 11; acyl-coenzyme A thioesterase 11; START domain containing 14; acyl-CoA thioester hydrolase 11; adipose-associated thioesterase; brown fat-inducible thioesterase; thioesterase, adipose associated; thioesterase superfamily member 1; StAR-related lipid transfer (START) domain containing 14; EC 3.1.2.-
• Gene ID: 26027
• mRNA Refseq: NM_015547
• Protein Refseq: NP_056362
• MIM: 606803
• UniProt ID: Q8WXI4
• Chromosome Location: 1p32.3
• Function: acyl-CoA hydrolase activity; carboxylesterase activity

Q&As

Can ACOT11 protein be used in drug development?

Yes, ACOT11 protein is a potential target for drug development, especially for metabolic disorders and cancer.

Can ACOT11 protein be targeted for drug development?

Yes, ACOT11 protein is a potential target for drug development in metabolic disorders, cancer research, and other areas. Targeting its activity could have therapeutic benefits for various diseases and conditions.

Is ACOT11 protein used in diagnostic tests?

ACOT11 protein is not commonly used in diagnostic tests, but changes in its expression or activity may serve as biomarkers for certain diseases or conditions.

What are the potential applications of ACOT11 protein?

ACOT11 protein may have potential applications in the treatment of metabolic disorders such as obesity and diabetes, as well as in cancer research and sports nutrition.

How is ACOT11 protein different from other fatty acid metabolism enzymes?

ACOT11 protein is a specific type of enzyme that is involved in the breakdown of long-chain fatty acids. It has a unique structure and function compared to other enzymes involved in fatty acid metabolism, such as lipases and acyl-CoA synthetases.

How can ACOT11 protein be used in cancer research?

ACOT11 protein may have potential anticancer effects by regulating cancer cell metabolism and inducing cell death in cancer cells.

What applications does ACOT11 protein have in medical research?

ACOT11 protein may have therapeutic implications for the treatment of metabolic disorders such as obesity and diabetes, as well as certain types of cancer. It may also be a target for drug development to enhance fatty acid oxidation.

How can ACOT11 protein be used to treat metabolic disorders?

ACOT11 protein can help to regulate lipid metabolism and reduce lipid accumulation in tissues, which can be beneficial in preventing and treating metabolic disorders such as obesity and diabetes.

What research areas are exploring the potential of ACOT11 protein?

Research areas exploring the potential of ACOT11 protein include metabolic disorders such as diabetes and obesity, as well as cancer research and drug development.

How is ACOT11 protein regulated in the body?

ACOT11 protein is regulated by various factors, including hormones, nutrients, and metabolic pathways. Its activity can be affected by insulin, glucagon, and other hormones involved in the regulation of lipid metabolism.

What challenges exist in targeting ACOT11 protein for drug development?

One challenge is understanding the complex role of ACOT11 in lipid metabolism and its potential off-target effects on other metabolic pathways. Developing selective ACOT11 inhibitors or modulators with good pharmacokinetic properties is also a challenge.

Is ACOT11 protein already being used in any treatments or products?

ACOT11 protein is still being studied and has not yet been approved for specific treatments or products. However, it has been identified as a potential target for drug development in metabolic disorders and cancer research.