Recombinant Human Casein Kinase 1, Gamma 2, GST-tagged

• Cat.No.: CSNK1G2-1008H
• Product Overview: Full length recombinant human CSNK1G2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: CSNK1G2-1008H
• Description: CSNK1G2 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CSNK1G2 is a ubiquitously expressed cytoplasmic kinase that can interact and phosphorylate the metastatic tumor antigen 1 short form (MTA1s) co-localizing it in the cytoplasm. Phosphorylated MTA1s then sequesters the estrogen receptor-alpha in the cytoplasm of breast cancer cells. CSNK1G2 hyperphosphorylates the ceramide transfer protein CERT leading to a decrease in de novo sphingomyelin synthesis. The reduction in synthesis of sphingomyelin is reversed by the expression of CERT mutants that are not hyperphosphorylated.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 73 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 30 nmol/min/mg
• Purity: > 80 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: FoxO family signaling; Hedgehog signaling events mediated by Gli proteins; Hedgehog signaling pathway; Metabolism of lipids and lipoproteins; Sphingolipid metabolism; p53 pathway

Gene Information

• Gene Name: CSNK1G2 casein kinase 1, gamma 2 [ Homo sapiens ]
• Official Symbol: CSNK1G2
• Synonyms: CSNK1G2; casein kinase 1, gamma 2; CK1g2; CKI-gamma 2; casein kinase 1 isoform gamma-2; casein kinase I isoform gamma-2; EC 2.7.11.1
• Gene ID: 1455
• mRNA Refseq: NM_001319
• Protein Refseq: NP_001310
• MIM: 602214
• UniProt ID: P78368
• Chromosome Location: 19p13.3
• Function: ATP binding; glycoprotein binding; magnesium ion binding; nucleotide binding; peptide binding; phosphoprotein binding; protein serine/threonine kinase activity; transferase activity