Active Recombinant Human CD274 protein, Avi-His-tagged, Biotinylated
Cat.No. : | CD274-1283H |
Product Overview : | Biotinylated Recombinant Human CD274 protein(Phe 19 - Arg 238), fused with Avi and His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | Avi-His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) with a linear range of 0.1-2 ng/mL. Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-3 ng/mL. |
Molecular Mass : | This protein carries an Avi tag (Avitag) at the C-terminus, followed by a polyhistidine tagThe protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Phe 19 - Arg 238 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | CD274 CD274 molecule [ Homo sapiens ] |
Official Symbol : | CD274 |
Synonyms : | CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
Gene ID : | 29126 |
mRNA Refseq : | NM_014143 |
Protein Refseq : | NP_054862 |
MIM : | 605402 |
UniProt ID : | Q9NZQ7 |
Products Types
◆ Recombinant Protein | ||
CD274-0765H | Recombinant Human CD274 Protein, GST-Tagged | +Inquiry |
CD274-199C | Recombinant Human CD274 Protein, His-tagged | +Inquiry |
CD274-710H | Recombinant Human CD274 Protein, IgG1 Fc-tagged | +Inquiry |
CD274-593H | Recombinant Human CD274 Protein | +Inquiry |
CD274-712M | Recombinant Mouse CD274 Protein | +Inquiry |
◆ Lysates | ||
CD274-914CCL | Recombinant Cynomolgus CD274 cell lysate | +Inquiry |
CD274-2642MCL | Recombinant Mouse CD274 cell lysate | +Inquiry |
CD274-002RCL | Recombinant Rat CD274 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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