| Description : |
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM. CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
C-His |
| Molecular Mass : |
~69 kDa |
| AA Sequence : |
QIDLNITCRFAGVFHVEKNGRYSIS RTEAADLCKAFNSTLPTMAQMEKAL SIGFETCRYGFIEGHVVIPRIHPNS ICAANNTGVYILTSNTSQYDTYCFN ASAPPEEDCTSVTDLPNAFDGPITI TIVNRDGTRYVQKGEYRTNPEDIYP SNPTDDDVSSGSSSERSSTSGGYIF YTFSTVHPIPDEDSPWITDSTDRIP ATTLMSTSATATETATKRQETWDWF SWLFLPSESKNHLHTTTQMAGTSSN TISAGWEPNEENEDERDRHLSFSGS GIDDDEDFISSTISTTPRAFDHTKQ NQDWTQWNPSHSNPEVLLQTTTRMT DVDRNGTTAYEGNWNPEAHPPLIHH EHHEEEETPHSTSTIQATPSSTTEE TATQKEQWFGNRWHEGYRQTPKEDS HSTTGTAAASAHTSHPMQGRTTPSP EDSSWTDFFNPISHPMGRGHQAGRR MDMDSSHSITLQPTANPNTGLVEDL DRTGPLSMTTQQSNSQSFSTSHEGL EEDKDHPTTSTLTSSNRNDVTGGRR DPNHSEGSTTLLEGYTSHYPHTKES RTFIPVTSAKTGSFGVTAVTVGDSN SNVNRSLSGDQDTFHPSGGSHTTHG SESDGHSHGSQEGGANTTSGPIRTP QIPE |
| Purity : |
Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE). |
| Notes : |
For research use only, not for use in diagnostic procedure. |
| Storage : |
Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles. |
| Concentration : |
≥0.5 mg/mL |
| Storage Buffer : |
PBS, 4M Urea, pH7.4 |
