Recombinant Human CDK4 protein, His/T7-tagged

• Cat.No.: CDK4-938H
• Product Overview: Recombinant Human CDK4(Met1-Glu303) fused with His/T7 tag at N-terminal was expressed in E. coli.

Specification

• Description: Cyclin-Dependent Kinase 4 (CDK4) is a member of the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK4 is a component of Cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. These complexes are major integrators of various mitogenenic and antimitogenic signals. It is shown that CDK4 is responsible for the phosphorylation of retinoblastoma gene product (Rb). Defects in CDK4 are a cause of susceptibility to cutaneous Malignant Melanoma Type 3.
• Source: E. coli
• Species: Human
• Tag: His/T7
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
• Protein length: Met1-Glu303
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMASMT GGQQMGRGSMATSRYEPVAEIGVGA YGTVYKARDPHSGHF VALKSVRVPNGGGGGGGLPISTVRE VALLRRLEAFEHPNVVRLMDVCATS RTDREIKVTLVFEHV DQDLRTYLDKAPPPGLPAETIKDLM RQFLRGLDFLHANCIVHRDLKPENI LVTSGGTVKLADFGL ARIYSYQMALTPVVVTLWYRAPEVL LQSTYATPVDMWSVGCIFAEMFRRK PLFCGNSEADQLGKI FDLIGLPPEDDWPRDVSLPRGAFPP RGPRPVQSVVPEMEESGAQLLLEML TFNPHKRISAFRALQ HSYLHKDEGNPE
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100 μg/ml.
  Dissolve the lyophilized protein in ddH2O.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ]
• Official Symbol: CDK4
• Synonyms: CDK4; cyclin-dependent kinase 4; PSK J3; cell division protein kinase 4; CMM3; PSK-J3; MGC14458
• Gene ID: 1019
• mRNA Refseq: NM_000075
• Protein Refseq: NP_000066
• MIM: 123829
• UniProt ID: P11802
• Chromosome Location: 12q13
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity

Reviews

02/18/2023

Whether it's troubleshooting experimental issues, providing guidance on experimental design, or answering any inquiries related to the CDK4 protein, the manufacturer is committed to helping researchers overcome challenges and achieve their scientific goals.

02/16/2020

They actively seek feedback and incorporate it into their product development process, continuously improving the quality and performance of the CDK4 protein.

05/29/2018

The manufacturer also values customer satisfaction and strives to maintain a strong relationship with researchers.

Q&As

Are there any potential challenges in targeting CDK4 for cancer therapy?

Resistance to CDK4 inhibitors and the need for patient stratification are some of the challenges in using CDK4 as a therapeutic target.

Can CDK4 be targeted for cancer therapy?

Yes, CDK4 is a target for cancer therapy, and drugs that inhibit CDK4 have been developed for the treatment of specific cancers.

How is CDK4 clinically relevant?

CDK4 is clinically relevant because it plays a crucial role in the development and progression of various cancers.

Which cancers are commonly associated with CDK4 overactivity?

CDK4 overactivity is commonly associated with breast cancer, melanoma, and liposarcoma.

How does CDK4 relate to cell senescence?

CDK4 can promote cell senescence, a state of irreversible growth arrest, which has implications in cancer therapy.