Recombinant Human CENPE Protein, GST-Tagged

• Cat.No.: CENPE-1114H
• Product Overview: Human CENPE partial ORF (NP_001804, 309 a.a. - 419 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 37.95 kDa
• AA Sequence: TPVSFDETLTALQFASTAKYMKNTP YVNEVSTDEALLKRYRKEIMDLKKQ LEEVSLETRAQAMEKDQLAQLLEEK DLLQKVQNEKIENLTRMLVTSSSLT LQQELKAKRKR
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CENPE centromere protein E, 312kDa [ Homo sapiens ]
• Official Symbol: CENPE
• Synonyms: CENPE; centromere protein E, 312kDa; centromere protein E (312kD); centromere-associated protein E; KIF10; PPP1R61; protein phosphatase 1; regulatory subunit 61; kinesin family member 10; kinesin-related protein CENPE; Centromere autoantigen E (312kD); protein phosphatase 1, regulatory subunit 61; CENP-E
• Gene ID: 1062
• mRNA Refseq: NM_001813
• Protein Refseq: NP_001804
• MIM: 117143
• UniProt ID: Q02224