Recombinant Human CENPE Protein, His-tagged

• Cat.No.: CENPE-348H
• Product Overview: Recombinant Human CENPE(Ala2450~Arg2590) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 20.2kDa
• Identity: Reconstitute in PBS or others
• Protein length: Ala2450~Arg2590
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: CENPE centromere protein E, 312kDa [ Homo sapiens ]
• Official Symbol: CENPE
• Synonyms: CENPE; centromere protein E, 312kDa; centromere protein E (312kD); centromere-associated protein E; KIF10; PPP1R61; protein phosphatase 1; regulatory subunit 61; kinesin family member 10; kinesin-related protein CENPE; Centromere autoantigen E (312kD); protein phosphatase 1, regulatory subunit 61; CENP-E
• Gene ID: 1062
• mRNA Refseq: NM_001813
• Protein Refseq: NP_001804
• MIM: 117143
• UniProt ID: Q02224