Recombinant Human CFL2 293 Cell Lysate

• Cat.No.: CFL2-7554HCL

Specification

• Description: Antigen standard for cofilin 2 (muscle) (CFL2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: CFL2 cofilin 2 (muscle) [ Homo sapiens ]
• Official Symbol: CFL2
• Synonyms: CFL2; cofilin 2 (muscle); cofilin-2; cofilin, muscle isoform; NEM7
• Gene ID: 1073
• mRNA Refseq: NM_001243645
• Protein Refseq: NP_001230574
• MIM: 601443
• UniProt ID: Q9Y281
• Chromosome Location: 14q13.2
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Caspase cascade in apoptosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; G13 Signaling Pathway, organism-specific biosystem; Pertussis, organism-specific biosystem
• Function: actin binding; protein binding

Reviews

05/23/2022

It has been extensively used in protein-protein interaction studies, enzymatic assays, and structural analyses, showcasing its reliability and adaptability in diverse research areas.

11/13/2021

I am certain of obtaining reliable and reproducible results, enabling me to contribute to scientific progress and make new discoveries.

11/07/2017

I highly recommend the CFL2 Protein for its outstanding performance in ELISA assays.

Q&As

Are there any ongoing clinical trials targeting CFL2 for therapeutic purposes?

It's important to check current clinical trial databases for the latest information, but research exploring CFL2 as a therapeutic target is ongoing.

How does CFL2 relate to cardiac diseases?

CFL2 is involved in maintaining the structure and function of cardiac muscle cells, and its dysregulation has been linked to conditions such as cardiomyopathies.

Are there diagnostic applications for CFL2 in muscle-related disorders?

Research suggests that analyzing CFL2 levels and activity could serve as diagnostic markers for certain muscle-related disorders.

How does CFL2 interact with other proteins in the context of muscle function?

CFL2 interacts with actin and other regulatory proteins to modulate the dynamics of the actin cytoskeleton, influencing cellular processes crucial for muscle function.

Can CFL2 be a potential therapeutic target for muscle-related disorders?

Investigating CFL2 as a therapeutic target is an active area of research, with the aim of developing interventions for conditions characterized by impaired muscle function.