Recombinant Human COL17A1 293 Cell Lysate

• Cat.No.: COL17A1-7378HCL

Specification

• Description: Antigen standard for collagen, type XVII, alpha 1 (COL17A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: COL17A1 collagen, type XVII, alpha 1 [ Homo sapiens ]
• Official Symbol: COL17A1
• Synonyms: COL17A1; collagen, type XVII, alpha 1; BPAG2; collagen alpha-1(XVII) chain; BP180; alpha 1 type XVII collagen; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BPA-2; LAD-1; BA16H23.2; FLJ60881; KIAA0204
• Gene ID: 1308
• mRNA Refseq: NM_000494
• Protein Refseq: NP_000485
• MIM: 113811
• UniProt ID: Q9UMD9
• Chromosome Location: 10q24.3
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Type I hemidesmosome assembly, organism-specific biosystem; a6b1 and a6b4 Integrin signaling, organism-specific biosystem
• Function: protein binding

Reviews

03/10/2022

By incorporating the COL17A1 Protein into their studies, scientists have the opportunity to conduct their research with utmost confidence, paving the way for groundbreaking discoveries and novel insights in their respective fields.

12/06/2021

Considering the aforementioned qualities, I wholeheartedly recommend the COL17A1 Protein to fellow researchers in search of a top-tier protein solution that not only meets their experimental needs but also benefits from exceptional technical support.

05/02/2016

What further sets the COL17A1 Protein apart is the outstanding technical support provided by the manufacturer.

Q&As

Are there any known interactions between COL17A1 protein and other cellular components?

COL17A1 protein interacts with integrins, laminins, and other extracellular matrix proteins to maintain skin integrity.

How does COL17A1 protein contribute to the pathogenesis of autoimmune skin diseases?

Dysregulation of COL17A1 protein can trigger autoimmunity and lead to the development of autoimmune skin diseases such as bullous pemphigoid.

What are the potential therapeutic targets related to COL17A1 protein?

Are there any known interactions between COL17A1 protein and other cellular components?

Are there any clinical implications of COL17A1 mutations beyond skin disorders?

Mutations in COL17A1 have been associated with certain forms of cancer, indicating a potential role in tumor progression and metastasis.

How is COL17A1 protein measured in a clinical setting?

COL17A1 protein levels can be measured using techniques such as western blotting, ELISA, and immunohistochemistry.