Recombinant Human COL17A1 Protein, His-tagged

• Cat.No.: COL17A1-329H
• Product Overview: Recombinant Human COL17A1(Pro184~Pro375) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 21.5kDa
• Protein length: Pro184~Pro375
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: COL17A1 collagen, type XVII, alpha 1 [ Homo sapiens ]
• Official Symbol: COL17A1
• Synonyms: COL17A1; collagen, type XVII, alpha 1; BPAG2; collagen alpha-1(XVII) chain; BP180; alpha 1 type XVII collagen; collagen XVII, alpha-1 polypeptide; 180 kDa bullous pemphigoid antigen 2; bullous pemphigoid antigen 2 (180kD); bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BPA-2; LAD-1; BA16H23.2; FLJ60881; KIAA0204
• Gene ID: 1308
• mRNA Refseq: NM_000494
• Protein Refseq: NP_000485
• MIM: 113811
• UniProt ID: Q9UMD9

Reviews

03/10/2022

By incorporating the COL17A1 Protein into their studies, scientists have the opportunity to conduct their research with utmost confidence, paving the way for groundbreaking discoveries and novel insights in their respective fields.

12/06/2021

Considering the aforementioned qualities, I wholeheartedly recommend the COL17A1 Protein to fellow researchers in search of a top-tier protein solution that not only meets their experimental needs but also benefits from exceptional technical support.

05/02/2016

What further sets the COL17A1 Protein apart is the outstanding technical support provided by the manufacturer.

Q&As

Are there any known interactions between COL17A1 protein and other cellular components?

COL17A1 protein interacts with integrins, laminins, and other extracellular matrix proteins to maintain skin integrity.

How does COL17A1 protein contribute to the pathogenesis of autoimmune skin diseases?

Dysregulation of COL17A1 protein can trigger autoimmunity and lead to the development of autoimmune skin diseases such as bullous pemphigoid.

What are the potential therapeutic targets related to COL17A1 protein?

Are there any known interactions between COL17A1 protein and other cellular components?

Are there any clinical implications of COL17A1 mutations beyond skin disorders?

Mutations in COL17A1 have been associated with certain forms of cancer, indicating a potential role in tumor progression and metastasis.

How is COL17A1 protein measured in a clinical setting?

COL17A1 protein levels can be measured using techniques such as western blotting, ELISA, and immunohistochemistry.