Recombinant Human CSF3R protein(Met1-Pro621)

• Cat.No.: CSF3R-1041H
• Product Overview: Recombinant Human G-CSF receptor (NP_000751.1)(Met 1-Pro 621) with five aminio acids at the C-terminus was expressed and purified in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Protein length: Met1-Pro621
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit GCSF-induced proliferation of NFS60 mouse myeloid cells.The ED50 for this effect is typically 2-8 μg/mL.
• Molecular Mass: The mature recombinant human G-CSFR consists of 603 amino acids and predicts a molecular mass of 69.0 KDa. As a result of glycosylation, the apparent molecular mass of rhG-CSFR is approximately 94 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CSF3R colony stimulating factor 3 receptor (granulocyte) [ Homo sapiens ]
• Official Symbol: CSF3R
• Synonyms: CSF3R; colony stimulating factor 3 receptor (granulocyte); CD114; granulocyte colony-stimulating factor receptor; GCSFR; G-CSF-R; CD114 antigen; G-CSF receptor
• Gene ID: 1441
• mRNA Refseq: NM_000760
• Protein Refseq: NP_000751
• MIM: 138971
• UniProt ID: Q99062