Recombinant Human CYP1A2/NADPH protein, Active

• Cat.No.: CYP1A2-361H
• Product Overview: This is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP1A2 and recombinant human P450 NADPH oxidoreductase co expressed in the same preparation.

Specification

• Description: Cytochrome P450 1A2 (CYP1A2, EC 1.14.14.1) is a member of the cytochrome P450 monooxidase (CYP) family of microsomal xenobiotic metabolism enzymes. CYPs are membrane-bound hemeproteins responsible for Phase I biotransformation reactions, in which lipophilic drugs and other xenobiotic compounds are converted to more hydrophilic products to facilitate excretion from the body. CYP1A2 metabolizes many antidepressants and antipsychotic drugs, some of its substrates being caffeine, acetaminophen, fluvoxamine and Haloperidol. Expression of CYP1A2 appears to be induced by various dietary constituents like cabbages, cauliflower and broccoli. BioVision’s EZCypTM 1A2 is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP1A2 and recombinant human P450 NADPH oxidoreductase co expressed in the same preparation.
• Source: Pichia pastoris
• Species: Human
• Form: Lyophilized powder
• Molecular Mass: 55 Kda
• Notes: Centrifuge the vial prior to opening
• Storage: Stable for 1 year as supplied. Store dry powder at -20 centigrade. Reconstitute in 100 mM Potassium Phosphate buffer, pH 7.7. Not recommended to be stored as solution. If storing as solution, aliquot and store at -80 centigrade. Avoid repeated freeze/thaw cycles and use aliquots within one month (the human CYP1A2 will lose approximately 10% activity per week when stored at -80 centigrade). Thaw aliquots rapidly at 37 centigrade and place on ice until use (thawed aliquots should be used within 4 hours).
• Reconstitution: Reconstitute in 100 mM Potassium Phosphate buffer, pH 7.7.

Gene Information

• Gene Name: CYP1A2 cytochrome P450, family 1, subfamily A, polypeptide 2 [ Homo sapiens ]
• Official Symbol: CYP1A2
• Synonyms: CYP1A2; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound inducible), polypeptide 2; cytochrome P450 1A2; CP12; P3 450; CYPIA2; P450 form 4; cytochrome P450 4; cytochrome P(3)450; cytochrome P450-P3; dioxin-inducible P3-450; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; P3-450; P450(PA)
• Gene ID: 1544
• mRNA Refseq: NM_000761
• Protein Refseq: NP_000752
• MIM: 124060
• UniProt ID: P05177
• Chromosome Location: 15q24.1
• Pathway: Aromatic amines can be N-hydroxylated or N-dealkylated by CYP1A2, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Caffeine metabolism, organism-specific biosystem; Caffeine metabolism, conserved biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem
• Function: aromatase activity; caffeine oxidase activity; demethylase activity; electron carrier activity; enzyme binding; heme binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen

Reviews

10/15/2022

Enables data-driven decisions. Elevates research standards.

08/02/2021

Invaluable for insights. Highly recommended for research.

10/23/2020

Swift and accurate service. Supports research credibility.

Q&As

How does CYP1A2 interact with other cytochrome P450 enzymes in metabolic pathways?

CYP1A2 collaborates with other P450 enzymes in metabolic processes.

What is the impact of altered CYP1A2 activity on liver function and disease risk?

Changes in CYP1A2 activity can affect liver health and disease risk.

How do genetic variations in CYP1A2 affect drug metabolism and response?

Genetic differences in CYP1A2 influence drug metabolism and individual medication responses.

What is the primary role of CYP1A2 in xenobiotic metabolism?

CYP1A2 is key in metabolizing drugs and environmental chemicals.

What role does CYP1A2 play in the detoxification of environmental toxins?

CYP1A2 helps detoxify harmful environmental substances.

How does CYP1A2 contribute to the metabolism of caffeine and other dietary compounds?

It metabolizes caffeine and certain dietary compounds, affecting their bodily impact.

What potential therapeutic applications arise from understanding CYP1A2 activity in personalized medicine?

CYP1A2's role can inform personalized medicine, tailoring drug therapies to individual metabolic profiles.