Recombinant Human DDX4 293 Cell Lysate

• Cat.No.: DDX4-7006HCL

Specification

• Description: Antigen standard for DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 [ Homo sapiens ]
• Official Symbol: DDX4
• Synonyms: DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 4; probable ATP-dependent RNA helicase DDX4; VASA; vasa homolog; DEAD box protein 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074
• Gene ID: 54514
• mRNA Refseq: NM_001142549
• Protein Refseq: NP_001136021
• MIM: 605281
• UniProt ID: Q9NQI0
• Chromosome Location: 5p15.2-p13.1
• Function: ATP binding; ATP-dependent helicase activity; helicase activity; hydrolase activity; nucleic acid binding; nucleotide binding

Reviews

08/19/2019

Their prompt assistance and willingness to go above and beyond to ensure my success have been immensely valuable.

05/09/2018

This protein has proven to be an invaluable asset in my experiments, enabling accurate and precise measurements with exceptional sensitivity.

10/24/2017

Its purity, stability, and consistency are unparalleled, ensuring reliable and reproducible results in my research.

Q&As

How is DDX4 protein expression used in clinical diagnosis?

DDX4 protein expression is used as a biomarker for germ cell development and certain reproductive diseases, aiding in diagnosis and prognosis.

Does DDX4 protein have a role in the development of male and female gametes?

Yes, DDX4 protein plays an essential role in the development of both male and female gametes, making it a crucial factor in reproductive health.

How does DDX4 protein influence oocyte and embryo development?

DDX4 protein is involved in the regulation of oocyte and embryo development, and its dysregulation can impact fertility and reproductive outcomes.

How is DDX4 protein expression assessed in clinical settings?

DDX4 protein expression can be assessed using immunohistochemistry, western blotting, and other molecular techniques in clinical settings.

Are there any known genetic mutations affecting DDX4 protein associated with clinical conditions?

Yes, genetic mutations in DDX4 protein have been linked to certain forms of infertility and germ cell tumors.