Recombinant Human DFFA

• Cat.No.: DFFA-488H
• Product Overview: Recombinant DFFA protein was expressed inE.coliand purified by using conventional chromatography techniques. MW=38.7 kDa (351aa).

Specification

• Cat. No.: DFFA-488H
• Description: DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: DFFA DNA fragmentation factor, 45kDa, alpha polypeptide [ Homo sapiens ]
• Synonyms: DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; DFF1; ICAD; DFF-45; DFF45; ICAD; DFF1; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; DFF45; H13; OTTHUMP00000001903; OTTHUMP00000001904; DNA fragmentation factor, 45 kD, alpha subunit
• Gene ID: 1676
• mRNA Refseq: NM_004401
• Protein Refseq: NP_004392
• MIM: 601882
• UniProt ID: O00273
• Chromosome Location: 1p36.3-p36.2
• Pathway: Apoptosis
• Function: caspase-activated deoxyribonuclease activity; identical protein binding

Reviews

12/23/2021

We felt well-supported throughout our usage of the product, thanks to the dedicated customer service.

10/18/2019

Consistency in the product's behavior across experimental conditions enhanced the credibility of our findings.

05/11/2019

The product's adaptability to various buffers contributed to its stable and reliable performance.

Q&As

What diseases or conditions are associated with dysregulation or abnormal expression of DFFA, particularly in the context of apoptotic dysfunction?

Dysregulation or abnormal expression of DFFA is associated with various conditions, including cancer and neurodegenerative disorders, highlighting its role in apoptotic dysfunction.

How does DFFA contribute to DNA fragmentation and chromatin condensation during apoptotic cell death?

DFFA contributes to DNA fragmentation and chromatin condensation by being cleaved and activated by caspases, particularly caspase-3, during apoptotic cell death.

In which cellular compartments is DFFA predominantly localized, especially during the initiation of apoptosis?

DFFA is predominantly localized in the nucleus, specifically during the initiation of apoptosis, where it actively participates in chromatin condensation and DNA fragmentation.

Are there therapeutic implications or potential targets related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival?

There are potential therapeutic implications related to DFFA in diseases involving apoptotic dysregulation and abnormal cell survival, making it a target for drug development.

What is the primary function of DNA Fragmentation Factor Subunit Alpha (DFFA) in cellular processes and apoptosis?

DNA Fragmentation Factor Subunit Alpha (DFFA) plays a crucial role in cellular processes, primarily contributing to the execution of apoptosis and programmed cell death.

How does DFFA participate in regulating the balance between cell survival and programmed cell death in various tissues?

DFFA actively participates in regulating the balance between cell survival and programmed cell death in various tissues, influencing tissue homeostasis.

Are there specific caspases or factors that activate DFFA, influencing its role in the execution phase of apoptosis?

Specific caspases, including caspase-3, activate DFFA, influencing its role in the execution phase of apoptosis and facilitating efficient DNA degradation.