Recombinant Human DHODH, MYC/DDK-tagged

• Cat.No.: DHODH-4839H
• Product Overview: Recombinant Human DHODH fused with C-terminal MYC/DDK, was expressed in Human 293HEK cells.

Specification

• Description: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
• Source: Human 293HEK cells
• Species: Human
• Tag: MYC/DDK
• Molecular Mass: 41.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: DHODH dihydroorotate dehydrogenase (quinone) [ Homo sapiens (human) ]
• Official Symbol: DHODH
• Synonyms: DHODH; URA1; POADS; DHOdehase; dihydroorotate dehydrogenase (quinone); dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate oxidase; human complement of yeast URA1; NP_001352.2; EC 1.3.5.2
• Gene ID: 1723
• mRNA Refseq: NM_001361
• Protein Refseq: NP_001352
• MIM: 126064
• UniProt ID: Q02127
• Chromosome Location: 16q22
• Pathway: Metabolism; Pyrimidine biosynthesis; Pyrimidine metabolism; Uridine monophosphate biosynthesis, glutamine (+ PRPP) => UMP
• Function: FMN binding; dihydroorotate dehydrogenase activity; dihydroorotate oxidase activity

Reviews

07/29/2020

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11/03/2017

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05/05/2017

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Q&As

How does DHODH contribute to pyrimidine biosynthesis?

It catalyzes a key step in the pyrimidine biosynthesis pathway, converting dihydroorotate to orotate.

What is the primary role of DHODH in cellular metabolism?

DHODH, dihydroorotate dehydrogenase, is crucial for the de novo synthesis of pyrimidines, essential for DNA and RNA synthesis.

How does DHODH interact with other enzymes in the pyrimidine biosynthesis pathway?

DHODH collaborates with other enzymes in pyrimidine biosynthesis, playing a role in nucleotide metabolism.

What role does DHODH play in cell proliferation and growth?

DHODH is important for cell proliferation, as pyrimidine synthesis is essential for DNA replication and cell growth.

How do genetic variations in DHODH affect its function in nucleotide synthesis?

Genetic mutations in DHODH can disrupt nucleotide synthesis, affecting cellular replication and function.

What is the impact of altered DHODH activity on autoimmune diseases?

Altered DHODH activity is linked with autoimmune conditions, as it affects lymphocyte proliferation and function.

What potential therapeutic applications arise from targeting DHODH in disease treatment?

Targeting DHODH in therapies could be effective in treating autoimmune diseases and certain cancers by modulating pyrimidine synthesis.