Recombinant Human DHODH Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DHODH-6058H
• Product Overview: DHODH MS Standard C13 and N15-labeled recombinant protein (NP_001352) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 42.9 kDa
• AA Sequence: MAWRHLKKRAQDAVIILGGGGLLFA SYLMATGDERFYAEHLMPTLQGLLD PESAHRLAVRFTSLGLLPRARFQDS DMLEVRVLGHKFRNPVGIAAGFDKH GEAVDGLYKMGFGFVEIGSVTPKPQ EGNPRPRVFRLPEDQAVINRYGFNS HGLSVVEHRLRARQQKQAKLTEDGL PLGVNLGKNKTSVDAAEDYAEGVRV LGPLADYLVVNVSSPNTAGLRSLQG KAELRRLLTKVLQERDGLRRVHRPA VLVKIAPDLTSQDKEDIASVVKELG IDGLIVTNTTVSRPAGLQGALRSET GGLSGKPLRDLSTQTIREMYALTQG RVPIIGVGGVSSGQDALEKIRAGAS LVQLYTALTFWGPPVVGKVKRELEA LLKEQGFGGVTDAIGADHRRTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DHODH dihydroorotate dehydrogenase (quinone) [ Homo sapiens (human) ]
• Official Symbol: DHODH
• Synonyms: DHODH; dihydroorotate dehydrogenase (quinone); dihydroorotate dehydrogenase; dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate oxidase; human complement of yeast URA1; URA1; POADS; DHOdehase
• Gene ID: 1723
• mRNA Refseq: NM_001361
• Protein Refseq: NP_001352
• MIM: 126064
• UniProt ID: Q02127

Reviews

07/29/2020

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11/03/2017

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05/05/2017

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Q&As

How does DHODH contribute to pyrimidine biosynthesis?

It catalyzes a key step in the pyrimidine biosynthesis pathway, converting dihydroorotate to orotate.

What is the primary role of DHODH in cellular metabolism?

DHODH, dihydroorotate dehydrogenase, is crucial for the de novo synthesis of pyrimidines, essential for DNA and RNA synthesis.

How does DHODH interact with other enzymes in the pyrimidine biosynthesis pathway?

DHODH collaborates with other enzymes in pyrimidine biosynthesis, playing a role in nucleotide metabolism.

What role does DHODH play in cell proliferation and growth?

DHODH is important for cell proliferation, as pyrimidine synthesis is essential for DNA replication and cell growth.

How do genetic variations in DHODH affect its function in nucleotide synthesis?

Genetic mutations in DHODH can disrupt nucleotide synthesis, affecting cellular replication and function.

What is the impact of altered DHODH activity on autoimmune diseases?

Altered DHODH activity is linked with autoimmune conditions, as it affects lymphocyte proliferation and function.

What potential therapeutic applications arise from targeting DHODH in disease treatment?

Targeting DHODH in therapies could be effective in treating autoimmune diseases and certain cancers by modulating pyrimidine synthesis.