Recombinant Human Dihydroorotate Dehydrogenase (quinone), His-tagged

• Cat.No.: DHODH-2486H
• Product Overview: Recombinant human DHODH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: DHODH belongs to the dihydroorotate dehydrogenase family. DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Mass: 42.3 kDa (390aa) confirmed by MALDI-TOF
• Protein length: 31-395aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTGDER FYAEHLMPTL QGLLDPESAH RLAVRFTSLG LLPRARFQDS DMLEVRVLGH KFRNPVGIAA GFDKHGEAVD GLYKMGFGFV EIGSVTPKPQ EGNPRPRVFR LPEDQAVINR YGFNSHGLSV VEHRLRARQQ KQAKLTEDGL PLGVNLGKNK TSVDAAEDYA EGVRVLGPLA DYLVVNVSSP NTAGLRSLQG KAELRRLLTK VLQERDGLRR VHRPAVLVKI APDLTSQDKE DIASVVKELG IDGLIVTNTT VSRPAGLQGA LRSETGGLSG KPLRDLSTQT IREMYALTQG RVPIIGVGGV SSGQDALEKI RAGASLVQLY TALTFWGPPV VGKVKRELEA LLKEQGFGGV TDAIGADHRR
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -77°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Publication:
  Dependence on the Pyrimidine Biosynthetic Enzyme DHODH Is a Synthetic Lethal Vulnerability in Mutant KRAS-Driven Cancers (2018)

Gene Information

• Gene Name: DHODH dihydroorotate dehydrogenase (quinone) [ Homo sapiens ]
• Official Symbol: DHODH
• Synonyms: DHODH; dihydroorotate dehydrogenase (quinone); dihydroorotate dehydrogenase; dihydroorotate dehydrogenase (quinone), mitochondrial; dihydroorotate oxidase; human complement of yeast URA1; URA1; POADS; DHOdehase
• Gene ID: 1723
• mRNA Refseq: NM_001361
• Protein Refseq: NP_001352
• MIM: 126064
• UniProt ID: Q02127
• Chromosome Location: 16q22
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Pyrimidine biosynthesis, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem
• Function: dihydroorotate dehydrogenase activity; dihydroorotate dehydrogenase activity; dihydroorotate oxidase activity; oxidoreductase activity

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07/29/2020

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Q&As

How does DHODH contribute to pyrimidine biosynthesis?

It catalyzes a key step in the pyrimidine biosynthesis pathway, converting dihydroorotate to orotate.

What is the primary role of DHODH in cellular metabolism?

DHODH, dihydroorotate dehydrogenase, is crucial for the de novo synthesis of pyrimidines, essential for DNA and RNA synthesis.

How does DHODH interact with other enzymes in the pyrimidine biosynthesis pathway?

DHODH collaborates with other enzymes in pyrimidine biosynthesis, playing a role in nucleotide metabolism.

What role does DHODH play in cell proliferation and growth?

DHODH is important for cell proliferation, as pyrimidine synthesis is essential for DNA replication and cell growth.

How do genetic variations in DHODH affect its function in nucleotide synthesis?

Genetic mutations in DHODH can disrupt nucleotide synthesis, affecting cellular replication and function.

What is the impact of altered DHODH activity on autoimmune diseases?

Altered DHODH activity is linked with autoimmune conditions, as it affects lymphocyte proliferation and function.

What potential therapeutic applications arise from targeting DHODH in disease treatment?

Targeting DHODH in therapies could be effective in treating autoimmune diseases and certain cancers by modulating pyrimidine synthesis.