Recombinant Human DSG1 protein, His & GST-tagged

• Cat.No.: DSG1-820H
• Product Overview: Recombinant Human DSG1 aa. (Glu710~Val961) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 56kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Glu710~Val961
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DSG1 desmoglein 1 [ Homo sapiens (human) ]
• Official Symbol: DSG1
• Synonyms: DG1; DSG; CDHF4; EPKHE; PPKS1; SPPK1; EPKHIA; cadherin family member 4; desmosomal glycoprotein 1; pemphigus foliaceus antigen; desmoglein-1
• Gene ID: 1828
• mRNA Refseq: NM_001942.3
• Protein Refseq: NP_001933.2
• UniProt ID: Q02413

Reviews

02/22/2023

Its impeccable purity and integrity make it a valuable asset in my research endeavors.

03/02/2022

Their team of experts has been instrumental in resolving any queries or challenges I encountered regarding the application and utilization of the DSG1 protein.

03/22/2021

The DSG1 protein surpasses expectations in terms of quality and is an ideal match for my experimental requirements.

Q&As

How is DSG1 expression regulated in the skin?

Various factors, including cytokines and growth factors, can regulate the expression of DSG1 in skin cells.

Are there any clinical trials focused on DSG1 protein therapies?

Some clinical trials may be exploring DSG1-based therapies for skin disorders, but you should consult clinical trial databases for specific information.

What other medical conditions are associated with DSG1 protein?

DSG1 abnormalities are primarily linked to skin disorders, but some studies suggest potential connections to oral mucosa disorders.

Can DSG1 be used as a biomarker for skin cancer?

While DSG1 is not a direct biomarker for skin cancer, its expression levels may be altered in some skin cancers.

Can DSG1-targeted therapies be used to treat skin conditions?

Research is ongoing, but DSG1-targeted therapies have the potential to treat conditions related to DSG1 deficiencies.